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Bacterial Cellulose-Binding Domain Modulates in Vitro Elongation of Different Plant Cells1

Etai Shpigel, Levava Roiz, Raphael Goren, and Oded Shoseyov*

The Kennedy Leigh Center for Horticulture Research and The Otto Warburg Center for Agricultural Biotechnology, The Faculty of Agriculture, The Hebrew University of Jerusalem, P.O. Box 12, Rehovot 76100, Israel

Recombinant cellulose-binding domain (CBD) derived from the cellulolytic bacterium Clostridium cellulovorans was found to modulate the elongation of different plant cells in vitro. In peach (Prunus persica L.) pollen tubes, maximum elongation was observed at 50 µg mL-1 CBD. Pollen tube staining with calcofluor showed a loss of crystallinity in the tip zone of CBD-treated pollen tubes. At low concentrations CBD enhanced elongation of Arabidopsis roots. At high concentrations CBD dramatically inhibited root elongation in a dose-responsive manner. Maximum effect on root hair elongation was at 100 µg mL-1, whereas root elongation was inhibited at that concentration. CBD was found to compete with xyloglucan for binding to cellulose when CBD was added first to the cellulose, before the addition of xyloglucan. When Acetobacter xylinum L. was used as a model system, CBD was found to increase the rate of cellulose synthase in a dose-responsive manner, up to 5-fold compared with the control. Electron microscopy examination of the cellulose ribbons produced by A. xylinum showed that CBD treatment resulted in a splayed ribbon composed of separate fibrillar subunits, compared with a thin, uniform ribbon in the control.

1   This study was supported in part by an Eshkol fellowship (to E.S.).
*   Corresponding author; e-mail shoseyov{at}agri.huji.ac.il; fax 972-8-946-2283.

Plant Physiol. (1998) 117: 1185-1194
Copyright Clearance Center:   0032-0889/98/117//10
© 1998 American Society of Plant Physiologists




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