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Gibberellin Dose-Response Regulation of GA4 Gene Transcript Levels in Arabidopsis1

Rachel J. Cowling2, Yuji Kamiya, Hideharu Seto, and Nicholas P. Harberd*

Department of Molecular Genetics, John Innes Centre, Colney Lane, Norwich NR4 7UJ, United Kingdom (R.J.C., N.P.H.); and Laboratory for Plant Hormone Function, Frontier Research Program (Y.K.), and Plant Function Laboratory (H.S.), The Institute of Physical and Chemical Research (RIKEN), Wako-shi, Saitama 351-01, Japan

The gibberellins (GAs) are a complex family of diterpenoid compounds, some of which are potent endogenous regulators of plant growth. As part of a feedback control of endogenous GA levels, active GAs negatively regulate the abundance of mRNA transcripts encoding GA biosynthesis enzymes. For example, Arabidopsis GA4 gene transcripts encode GA 3beta -hydroxylase, an enzyme that catalyzes the conversion of inactive to active GAs. Here we show that active GAs regulate GA4 transcript abundance in a dose-dependent manner, and that down-regulation of GA4 transcript abundance is effected by GA4 (the product of 3beta -hydroxylation) but not by its immediate precursor GA9 (the substrate). Comparison of several different GA structures showed that GAs active in promoting hypocotyl elongation were also active in regulating GA4 transcript abundance, suggesting that similar GA:receptor and subsequent signal transduction processes control these two responses. It is interesting that these activities were not restricted to 3beta -hydroxylated GAs, being also exhibited by structures that were not 3beta -hydroxylated but that had another electronegative group at C-3. We also show that GA-mediated control of GA4 transcript abundance is disrupted in the GA-response mutants gai and spy-5. These observations define a sensitive homeostatic mechanism whereby plants may regulate their endogenous GA levels.

1   R.J.C. was supported by a John Innes Foundation Studentship. The work in N.P.H.'s laboratory was funded through a Biotechnology and Biological Sciences Research Council Core Strategic grant to the John Innes Centre, a Biotechnology and Biological Sciences Research Council Plant Molecular Biology grant (no. PG208/0600), and by the European Commission DG XII Biotechnology Program (contract no. BIO4-96-0621).
2   Present address: Laboratoire de Biologie Cellulaire, Institut National de la Recherche Agronomique, Route de Saint Cyr, 78026 Versailles cedex, France.
*   Corresponding author; e-mail harberd{at}bbsrc.ac.uk; fax 44-1603-505725.

Plant Physiol. (1998) 117: 1195-1203
Copyright Clearance Center:   0032-0889/98/117//09
© 1998 American Society of Plant Physiologists




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