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A Novel Gene, pmgA, Specifically Regulates Photosystem Stoichiometry in the Cyanobacterium Synechocystis Species PCC 6803 in Response to High Light1

Yukako Hihara, Kintake Sonoike, and Masahiko Ikeuchi*

Department of Life Sciences, Graduate School of Arts and Sciences, University of Tokyo, Komaba 3-8-1, Meguro-ku, Tokyo 153-8902, Japan (Y.H., M.I.); and Department of Biological Sciences, Graduate School of Science, University of Tokyo, Hongo 7-3-1, Bunkyo-ku, Tokyo 113-0033, Japan (K.S.)

Previously, we identified a novel gene, pmgA, as an essential factor to support photomixotrophic growth of Synechocystis species PCC 6803 and reported that a strain in which pmgA was deleted grew better than the wild type under photoautotrophic conditions. To gain insight into the role of pmgA, we investigated the mutant phenotype of pmgA in detail. When low-light-grown (20 µE m-2 s-1) cells were transferred to high light (HL [200µE m-2 s-1]), pmgA mutants failed to respond in the manner typically associated with Synechocystis. Specifically, mutants lost their ability to suppress accumulation of chlorophyll and photosystem I and, consequently, could not modulate photosystem stoichiometry. These phenotypes seem to result in enhanced rates of photosynthesis and growth during short-term exposure to HL. Moreover, mixed-culture experiments clearly demonstrated that loss of pmgA function was selected against during longer-term exposure to HL, suggesting that pmgA is involved in acquisition of resistance to HL stress. Finally, early induction of pmgA expression detected by reverse transcriptase-PCR upon the shift to HL led us to conclude that pmgA is the first gene identified, to our knowledge, as a specific regulatory factor for HL acclimation.

1   This work was supported by a Research Fellowship for Young Scientists from the Japan Society of the Promotion of Science (to Y.H.); by grants-in-aid for encouragement of young scientists (no. 09740590 to K.S.), for scientific research on priority areas (no. 07251204 to M.I.), and for scientific research C (no. 08836002 to M.I.) from the Ministry of Education, Science, and Culture, Japan; and by a grant for Scientific Research from the Human Frontier Science program (to M.I.).
*   Corresponding author; e-mail mikeuchi{at}ims.u-tokyo.ac.jp; fax 81-3-5454-4337.

Plant Physiol. (1998) 117: 1205-1216
Copyright Clearance Center:   0032-0889/98/117//12
© 1998 American Society of Plant Physiologists




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