Plant Physiol. Illumina
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via CrossRef
Right arrow Citing Articles via Web of Science (68)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Shannon, J. C.
Right arrow Articles by Liu, K.-C.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Shannon, J. C.
Right arrow Articles by Liu, K.-C.
Agricola
Right arrow Articles by Shannon, J. C.
Right arrow Articles by Liu, K.-C.

Brittle-1, an Adenylate Translocator, Facilitates Transfer of Extraplastidial Synthesized ADP-Glucose into Amyloplasts of Maize Endosperms1

Jack C. Shannon*, Fang-Mei Pien, Heping Cao2, and Kang-Chien Liu

Department of Horticulture, 102 Tyson Building, The Pennsylvania State University, University Park, Pennsylvania 16802

Amyloplasts of starchy tissues such as those of maize (Zea mays L.) function in the synthesis and accumulation of starch during kernel development. ADP-glucose pyrophosphorylase (AGPase) is known to be located in chloroplasts, and for many years it was generally accepted that AGPase was also localized in amyloplasts of starchy tissues. Recent aqueous fractionation of young maize endosperm led to the conclusion that 95% of the cellular AGPase was extraplastidial, but immunolocalization studies at the electron- and light-microscopic levels supported the conclusion that maize endosperm AGPase was localized in the amyloplasts. We report the results of two nonaqueous procedures that provide evidence that in maize endosperms in the linear phase of starch accumulation, 90% or more of the cellular AGPase is extraplastidial. We also provide evidence that the brittle-1 protein (BT1), an adenylate translocator with a KTGGL motif common to the ADP-glucose-binding site of starch synthases and bacterial glycogen synthases, functions in the transfer of ADP-glucose into the amyloplast stroma. The importance of the BT1 translocator in starch accumulation in maize endosperms is demonstrated by the severely reduced starch content in bt1 mutant kernels.

1   This work was supported by U.S. Department of Agriculture Competitive Grant no. 94-37306-0737.
2   Present address: Department of Biochemistry and Biophysics, 2154 Molecular Biology Building, Iowa State University, Ames, IA 50011.
*   Corresponding author; e-mail jshannon{at}psu.edu; fax 1-814-863-6139.

Plant Physiol. (1998) 117: 1235-1252
Copyright Clearance Center:   0032-0889/98/117//18
© 1998 American Society of Plant Physiologists




This article has been cited by other articles:


Home page
 J Exp BotHome page
S. Comparot-Moss and K. Denyer
The evolution of the starch biosynthetic pathway in cereals and other grasses
J. Exp. Bot., July 1, 2009; 60(9): 2481 - 2492.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
S. Kirchberger, M. Leroch, M. A. Huynen, M. Wahl, H. E. Neuhaus, and J. Tjaden
Molecular and Biochemical Analysis of the Plastidic ADP-glucose Transporter (ZmBT1) from Zea mays
J. Biol. Chem., August 3, 2007; 282(31): 22481 - 22491.
[Abstract] [Full Text] [PDF]

Home page
 Crop Sci.Home page
D. E. Bowen, E. J. Souza, M. J. Guttieri, V. Raboy, and J. Fu
A Low Phytic Acid Barley Mutation Alters Seed Gene Expression
Crop Sci., July 16, 2007; 47(S2): S-149 - S-159.
[Abstract] [Full Text] [PDF]

Home page
 J Exp BotHome page
C. G. Bowsher, E. F. A. L. Scrase-Field, S. Esposito, M. J. Emes, and I. J. Tetlow
Characterization of ADP-glucose transport across the cereal endosperm amyloplast envelope
J. Exp. Bot., April 1, 2007; 58(6): 1321 - 1332.
[Abstract] [Full Text] [PDF]

Home page
 J Exp BotHome page
S. Rosti, H. Rudi, K. Rudi, H.-G. Opsahl-Sorteberg, B. Fahy, and K. Denyer
The gene encoding the cytosolic small subunit of ADP-glucose pyrophosphorylase in barley endosperm also encodes the major plastidial small subunit in the leaves
J. Exp. Bot., November 1, 2006; 57(14): 3619 - 3626.
[Abstract] [Full Text] [PDF]

Home page
 J Exp BotHome page
Y. Balmer, W. H Vensel, F. M DuPont, B. B Buchanan, and W. J Hurkman
Proteome of amyloplasts isolated from developing wheat endosperm presents evidence of broad metabolic capability
J. Exp. Bot., April 1, 2006; 57(7): 1591 - 1602.
[Abstract] [Full Text] [PDF]

Home page
 Plant Cell PhysiolHome page
E. Baroja-Fernandez, E. Etxeberria, F. J. Munoz, M. T. Moran-Zorzano, N. Alonso-Casajus, P. Gonzalez, and J. Pozueta-Romero
An Important Pool of Sucrose Linked to Starch Biosynthesis is Taken up by Endocytosis in Heterotrophic Cells
Plant Cell Physiol., April 1, 2006; 47(4): 447 - 456.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
Y. Balmer, W. H. Vensel, N. Cai, W. Manieri, P. Schurmann, W. J. Hurkman, and B. B. Buchanan
A complete ferredoxin/thioredoxin system regulates fundamental processes in amyloplasts
PNAS, February 21, 2006; 103(8): 2988 - 2993.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
M. Leroch, S. Kirchberger, I. Haferkamp, M. Wahl, H. E. Neuhaus, and J. Tjaden
Identification and Characterization of a Novel Plastidic Adenine Nucleotide Uniporter from Solanum tuberosum
J. Biol. Chem., May 6, 2005; 280(18): 17992 - 18000.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
E. Baroja-Fernandez, F. J. Munoz, A. Zandueta-Criado, M. T. Moran-Zorzano, A. M. Viale, N. Alonso-Casajus, and J. Pozueta-Romero
Most of ADP{middle dot}glucose linked to starch biosynthesis occurs outside the chloroplast in source leaves
PNAS, August 31, 2004; 101(35): 13080 - 13085.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
N. J. Patron, B. Greber, B. F. Fahy, D. A. Laurie, M. L. Parker, and K. Denyer
The lys5 Mutations of Barley Reveal the Nature and Importance of Plastidial ADP-Glc Transporters for Starch Synthesis in Cereal Endosperm
Plant Physiology, August 1, 2004; 135(4): 2088 - 2097.
[Abstract] [Full Text] [PDF]

Home page
 J Exp BotHome page
A. P. M. Weber, J. Schneidereit, and L. M. Voll
Using mutants to probe the in vivo function of plastid envelope membrane metabolite transporters
J. Exp. Bot., June 1, 2004; 55(400): 1231 - 1244.
[Abstract] [Full Text] [PDF]

Home page
 Plant Cell PhysiolHome page
E. Baroja-Fernandez, F. J. Munoz, T. Saikusa, M. Rodriguez-Lopez, T. Akazawa, and J. Pozueta-Romero
Sucrose Synthase Catalyzes the de novo Production of ADPglucose Linked to Starch Biosynthesis in Heterotrophic Tissues of Plants
Plant Cell Physiol., May 15, 2003; 44(5): 500 - 509.
[Abstract] [Full Text] [PDF]

Home page
 J Exp BotHome page
M. J. Emes, C. G. Bowsher, C. Hedley, M. M. Burrell, E. S. F. Scrase-Field, and I. J. Tetlow
Starch synthesis and carbon partitioning in developing endosperm
J. Exp. Bot., January 3, 2003; 54(382): 569 - 575.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
E. Glawischnig, A. Gierl, A. Tomas, A. Bacher, and W. Eisenreich
Starch Biosynthesis and Intermediary Metabolism in Maize Kernels. Quantitative Analysis of Metabolite Flux by Nuclear Magnetic Resonance
Plant Physiology, December 1, 2002; 130(4): 1717 - 1727.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
A. Koller, M. P. Washburn, B. M. Lange, N. L. Andon, C. Deciu, P. A. Haynes, L. Hays, D. Schieltz, R. Ulaszek, J. Wei, et al.
From the Cover: Proteomic survey of metabolic pathways in rice
PNAS, September 3, 2002; 99(18): 11969 - 11974.
[Abstract] [Full Text] [PDF]

Home page
 Plant Cell PhysiolHome page
E. Baroja-Fernandez, F. J. Munoz, T. Akazawa, and J. Pozueta-Romero
Reappraisal of the Currently Prevailing Model of Starch Biosynthesis in Photosynthetic Tissues: A Proposal Involving the Cytosolic Production of ADP-Glucose by Sucrose Synthase and Occurrence of Cyclic Turnover of Starch in the Chloroplast
Plant Cell Physiol., December 1, 2001; 42(12): 1311 - 1320.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
E. M. Farre, A. Tiessen, U. Roessner, P. Geigenberger, R. N. Trethewey, and L. Willmitzer
Analysis of the Compartmentation of Glycolytic Intermediates, Nucleotides, Sugars, Organic Acids, Amino Acids, and Sugar Alcohols in Potato Tubers Using a Nonaqueous Fractionation Method
Plant Physiology, October 1, 2001; 127(2): 685 - 700.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
D. M. Beckles, J. Craig, and A. M. Smith
ADP-Glucose Pyrophosphorylase Is Located in the Plastid in Developing Tomato Fruit
Plant Physiology, May 1, 2001; 126(1): 261 - 266.
[Abstract] [Full Text]

Home page
 Plant Physiol.Home page
P. Geigenberger, C. Stamme, J. Tjaden, A. Schulz, P. W. Quick, T. Betsche, H. J. Kersting, and H. E. Neuhaus
Tuber Physiology and Properties of Starch from Tubers of Transgenic Potato Plants with Altered Plastidic Adenylate Transporter Activity
Plant Physiology, April 1, 2001; 125(4): 1667 - 1678.
[Abstract] [Full Text]

Home page
 Plant Physiol.Home page
D. M. Beckles, A. M. Smith, and T. ap Rees
A Cytosolic ADP-Glucose Pyrophosphorylase Is a Feature of Graminaceous Endosperms, But Not of Other Starch-Storing Organs
Plant Physiology, February 1, 2001; 125(2): 818 - 827.
[Abstract] [Full Text]

Home page
 Plant Physiol.Home page
E. M. Farré, P. Geigenberger, L. Willmitzer, and R. N. Trethewey
A Possible Role for Pyrophosphate in the Coordination of Cytosolic and Plastidial Carbon Metabolism within the Potato Tuber
Plant Physiology, June 1, 2000; 123(2): 681 - 688.
[Abstract] [Full Text]

Home page
 Plant Physiol.Home page
D. N.P. Doan, H. Rudi, and O.-A. Olsen
The Allosterically Unregulated Isoform of ADP-Glucose Pyrophosphorylase from Barley Endosperm Is the Most Likely Source of ADP-Glucose Incorporated into Endosperm Starch
Plant Physiology, November 1, 1999; 121(3): 965 - 975.
[Abstract] [Full Text]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
ASPB Publications PLANT PHYSIOLOGY® THE PLANT CELL
Copyright © 1998 by the American Society of Plant Biologists