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Expressed Sequence Tags from a Root-Hair-Enriched Medicago truncatula cDNA Library1

Peter A. Covitz2, Lucinda S. Smith, and Sharon R. Long*

Department of Biological Sciences (P.A.C., L.S.S., S.R.L.), and Howard Hughes Medical Institute (L.S.S., S.R.L.), Stanford University, Stanford, California 94305-5020

The root hair is a specialized cell type involved in water and nutrient uptake in plants. In legumes the root hair is also the primary site of recognition and infection by symbiotic nitrogen-fixing Rhizobium bacteria. We have studied the root hairs of Medicago truncatula, which is emerging as an increasingly important model legume for studies of symbiotic nodulation. However, only 27 genes from M. truncatula were represented in GenBank/EMBL as of October, 1997. We report here the construction of a root-hair-enriched cDNA library and single-pass sequencing of randomly selected clones. Expressed sequence tags (899 total, 603 of which have homology to known genes) were generated and made available on the Internet. We believe that the database and the associated DNA materials will provide a useful resource to the community of scientists studying the biology of roots, root tips, root hairs, and nodulation.

1   S.R.L. is an investigator of the Howard Hughes Medical Institute. Additional support for this project came from the Department of Energy, Energy Biosciences Program (contract no. DE-FG03-90ER20010). P.A.C. was a fellow of the Jane Coffin Childs Memorial Fund for Medical Research.
2   Present address: Incyte Pharmaceuticals, 3174 Porter Drive, Palo Alto, CA 94304.
*   Corresponding author; e-mail fa.srl{at}forsythe.stanford.edu; fax 1-650-725-8309.

Plant Physiol. (1998) 117: 1325-1332
Copyright Clearance Center:   0032-0889/98/117//08
© 1998 American Society of Plant Physiologists




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