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The Boron Requirement and Cell Wall Properties of
Growing and
Stationary Suspension-Cultured
Chenopodium album L. Cells1
Axel Fleischer,
Christine Titel, and
Rudolf Ehwald*
Humboldt-Universität zu Berlin,
Mathematisch-Naturwissenschaftliche Fakultät I, Institut
für Biologie, Invalidenstrasse 42, 10115 Berlin, Germany
Suspension-cultured
Chenopodium album L. cells are capable of continuous,
long-term growth on a boron-deficient medium. Compared with cultures
grown with boron, these cultures contained more enlarged and detached
cells, had increased turbidity due to the rupture of a small number of
cells, and contained cells with an increased cell wall pore size. These
characteristics were reversed by the addition of boric acid ( 7
µM) to the boron-deficient cells. C. album
cells grown in the presence of 100 µM boric acid entered the stationary phase when they were not subcultured, and remained viable for at least 3 weeks. The transition from the growth phase to
the stationary phase was accompanied by a decrease in the wall pore
size. Cells grown without boric acid or with 7 µM boric
acid were not able to reduce their wall pore size at the transition to
the stationary phase. These cells could not be kept viable in the
stationary phase, because they continued to expand and died as a result
of wall rupture. The addition of 100 µM boric acid
prevented wall rupture and the wall pore size was reduced to normal
values. We conclude that boron is required to maintain the normal pore
structure of the wall matrix and to mechanically stabilize the wall at
growth termination.
1
This research was supported by grant no. Eh
14471-1 from the Deutsche Forschungsgemeinschaft, Bonn, Germany.
*
Corresponding author; e-mail rudolf=ehwald{at}rz.huberlin.de; fax
49-30-20-93-8635.
Plant Physiol. (1998) 117: 1401-1410
Copyright Clearance Center: 0032-0889/98/117//10
© 1998 American Society of Plant Physiologists
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