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Transcription from Heterologous rRNA Operon Promoters in Chloroplasts Reveals Requirement for Specific Activating Factors1

Priya Sriraman, Daniel Silhavy2, and Pal Maliga*

Waksman Institute, Rutgers, The State University of New Jersey, 190 Frelinghuysen Road, Piscataway, New Jersey 08854-8020

The plastid rRNA (rrn) operon in chloroplasts of tobacco (Nicotiana tabacum), maize, and pea is transcribed by the plastid-encoded plastid RNA polymerase from a sigma 70-type promoter (P1). In contrast, the rrn operon in spinach (Spinacia oleracea) and mustard chloroplasts is transcribed from the distinct Pc promoter, probably also by the plastid-encoded plastid RNA polymerase. Primer-extension analysis reported here indicates that in Arabidopsis both promoters may be active. To understand promoter selection in the plastid rrn operon in the different species, we have tested transcription from the spinach rrn promoter in transplastomic tobacco and from the tobacco rrn promoter in transplastomic Arabidopsis. Our data suggest that transcription of the rrn operon depends on species-specific factors that facilitate transcription initiation by the general transcription machinery.

1   This research was supported by the National Science Foundation (grant no. MCB 96-30763) and the Monsanto Company. P.S. was the recipient of a Johanna and Charles Busch Predoctoral Fellowship Award.
2   Present address: Agricultural Biotechnology Center, Szentgyörgyi 4, Gödöllö 2101, Hungary.
*   Corresponding author; e-mail maliga{at}waksman.rutgers.edu; fax 1-732-445-5735.

Plant Physiol. (1998) 117: 1495-1499
Copyright Clearance Center:   0032-0889/98/117//05
© 1998 American Society of Plant Physiologists




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