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Delta pH-Dependent Photosystem II Fluorescence Quenching Induced by Saturating, Multiturnover Pulses in Red Algae1

Estelle Delphin, Jean-Claude Duval, Anne-Lise Etienne, and Diana Kirilovsky*

Photorégulation et Dynamique des Membranes Végétales, Unité de Recherche Associée 1810, Centre National de la Recherche Scientifique, École Normale Supérieure, 46 rue d'Ulm, 75230 Paris cedex 05, France

We have previously shown that in the red alga Rhodella violacea, exposure to continuous low intensities of light 2 (green light) or near-saturating intensities of white light induces a Delta pH-dependent PSII fluorescence quenching. In this article we further characterize this fluorescence quenching by using white, saturating, multiturnover pulses. Even though the pulses are necessary to induce the Delta pH and the quenching, the development of the latter occurred in darkness and required several tens of seconds. In darkness or in the light in the presence of 2,5-dibromo-3-methyl-6-isopropyl-p-benzoquinone, the dissipation of the quenching was very slow (more than 15 min) due to a low consumption of the Delta pH, which corresponds to an inactive ATP synthase. In contrast, under far-red illumination or in the presence of 3-(3,4-dichlorophenyl)-1,1'-dimethylurea (only in light), the fluorescence quenching relaxed in a few seconds. The presence of N,N'-dicyclohexyl carbodiimide hindered this relaxation. We propose that the quenching relaxation is related to the consumption of Delta pH by ATP synthase, which remains active under conditions favoring pseudolinear and cyclic electron transfer.

1   This work was supported by a grant from the Société de Secours des Amis des Sciences to E.D.
*   Corresponding author; e-mail kirilov{at}wotan.ens.fr; fax 33-1-44-32-39-35.

Plant Physiol. (1998) 118: 103-113
Copyright Clearance Center:   0032-0889/98/118//11
© 1998 American Society of Plant Physiologists




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