Plant Physiol. Drug Metab Dispos
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via CrossRef
Right arrow Citing Articles via Web of Science (10)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Delphin, E.
Right arrow Articles by Kirilovsky, D.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Delphin, E.
Right arrow Articles by Kirilovsky, D.
Agricola
Right arrow Articles by Delphin, E.
Right arrow Articles by Kirilovsky, D.

Delta pH-Dependent Photosystem II Fluorescence Quenching Induced by Saturating, Multiturnover Pulses in Red Algae1

Estelle Delphin, Jean-Claude Duval, Anne-Lise Etienne, and Diana Kirilovsky*

Photorégulation et Dynamique des Membranes Végétales, Unité de Recherche Associée 1810, Centre National de la Recherche Scientifique, École Normale Supérieure, 46 rue d'Ulm, 75230 Paris cedex 05, France

We have previously shown that in the red alga Rhodella violacea, exposure to continuous low intensities of light 2 (green light) or near-saturating intensities of white light induces a Delta pH-dependent PSII fluorescence quenching. In this article we further characterize this fluorescence quenching by using white, saturating, multiturnover pulses. Even though the pulses are necessary to induce the Delta pH and the quenching, the development of the latter occurred in darkness and required several tens of seconds. In darkness or in the light in the presence of 2,5-dibromo-3-methyl-6-isopropyl-p-benzoquinone, the dissipation of the quenching was very slow (more than 15 min) due to a low consumption of the Delta pH, which corresponds to an inactive ATP synthase. In contrast, under far-red illumination or in the presence of 3-(3,4-dichlorophenyl)-1,1'-dimethylurea (only in light), the fluorescence quenching relaxed in a few seconds. The presence of N,N'-dicyclohexyl carbodiimide hindered this relaxation. We propose that the quenching relaxation is related to the consumption of Delta pH by ATP synthase, which remains active under conditions favoring pseudolinear and cyclic electron transfer.

1   This work was supported by a grant from the Société de Secours des Amis des Sciences to E.D.
*   Corresponding author; e-mail kirilov{at}wotan.ens.fr; fax 33-1-44-32-39-35.

Plant Physiol. (1998) 118: 103-113
Copyright Clearance Center:   0032-0889/98/118//11
© 1998 American Society of Plant Physiologists




This article has been cited by other articles:


Home page
 Mol Biol EvolHome page
R. Frommolt, S. Werner, H. Paulsen, R. Goss, C. Wilhelm, S. Zauner, U. G. Maier, A. R. Grossman, D. Bhattacharya, and M. Lohr
Ancient Recruitment by Chromists of Green Algal Genes Encoding Enzymes for Carotenoid Biosynthesis
Mol. Biol. Evol., December 1, 2008; 25(12): 2653 - 2667.
[Abstract] [Full Text] [PDF]

Home page
 Plant Cell PhysiolHome page
H. Nabe, R. Funabiki, Y. Kashino, H. Koike, and K. Satoh
Responses to Desiccation Stress in Bryophytes and an Important Role of Dithiothreitol-Insensitive Non-Photochemical Quenching Against Photoinhibition in Dehydrated States
Plant Cell Physiol., November 1, 2007; 48(11): 1548 - 1557.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
M. Ritz, J.-C. Thomas, A. Spilar, and A.-L. Etienne
Kinetics of Photoacclimation in Response to a Shift to High Light of the Red Alga Rhodella violacea Adapted to Low Irradiance
Plant Physiology, August 1, 2000; 123(4): 1415 - 1426.
[Abstract] [Full Text]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
ASPB Publications PLANT PHYSIOLOGY® THE PLANT CELL
Copyright © 1998 by the American Society of Plant Biologists