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Metabolism of Indole-3-Acetic Acid in Arabidopsis1

Anders Östin, Mariusz Kowalyczk, Rishikesh P. Bhalerao, and Göran Sandberg*

Department of Forest Genetics and Plant Physiology, The Swedish University of Agricultural Sciences, S-901 83 Umeå, Sweden

The metabolism of indole-3-acetic acid (IAA) was investigated in 14-d-old Arabidopsis plants grown in liquid culture. After ruling out metabolites formed as an effect of nonsterile conditions, high-level feeding, and spontaneous interconversions, a simple metabolic pattern emerged. Oxindole-3-acetic acid (OxIAA), OxIAA conjugated to a hexose moiety via the carboxyl group, and the conjugates indole-3-acetyl aspartic acid (IAAsp) and indole-3-acetyl glutamate (IAGlu) were identified by mass spectrometry as primary products of IAA fed to the plants. Refeeding experiments demonstrated that none of these conjugates could be hydrolyzed back to IAA to any measurable extent at this developmental stage. IAAsp was further oxidized, especially when high levels of IAA were fed into the system, yielding OxIAAsp and OH-IAAsp. This contrasted with the metabolic fate of IAGlu, since that conjugate was not further metabolized. At IAA concentrations below 0.5 µM, most of the supplied IAA was metabolized via the OxIAA pathway, whereas only a minor portion was conjugated. However, increasing the IAA concentrations to 5 µM drastically altered the metabolic pattern, with marked induction of conjugation to IAAsp and IAGlu. This investigation used concentrations for feeding experiments that were near endogenous levels, showing that the metabolic pathways controlling the IAA pool size in Arabidopsis are limited and, therefore, make good targets for mutant screens provided that precautions are taken to avoid inducing artificial metabolism.

1   This work was financed by the Swedish Foundation for Strategic Research and the European Commission DG XII biotechnology program.
*   Corresponding author; e-mail goran.sandberg{at}genfys.slu.se; fax 46-90-786-5901.

Plant Physiol. (1998) 118: 285-296
Copyright Clearance Center:   0032-0889/98/118//12
© 1998 American Society of Plant Physiologists




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