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Hexose Transport in Growing Petunia Pollen Tubes and Characterization of a Pollen-Specific, Putative Monosaccharide Transporter1

Bauke Ylstra2, 3, Dolores Garrido3, Jacqueline Busscher, and Arjen J. van Tunen*

Department Cell Biology, Agricultural Research Department-Centre for Plant Breeding and Reproduction Research, P.O. Box 16, 6700 AA Wageningen, The Netherlands

We investigated the molecular and physiological processes of sugar uptake and metabolism during pollen tube growth and plant fertilization. In vitro germination assays showed that petunia (Petunia hybrida) pollen can germinate and grow not only in medium containing sucrose (Suc) as a carbon source, but also in medium containing the monosaccharides glucose (Glc) or fructose (Fru). Furthermore, high-performance liquid chromatography analysis demonstrated a rapid and complete conversion of Suc into equimolar amounts of Glc and Fru when pollen was cultured in a medium containing 2% Suc. This indicates the presence of wall-bound invertase activity and uptake of sugars in the form of monosaccharides by the growing pollen tube. A cDNA designated pmt1 (petunia monosaccharide transporter 1), which is highly homologous to plant monosaccharide transporters, was isolated from petunia. Pmt1 belongs to a small gene family and is expressed specifically in the male gametophyte, but not in any other vegetative or floral tissues. Pmt1 is activated after the first pollen mitosis, and high levels of mRNA accumulate in mature and germinating pollen. A model describing the transport of sugars to the style, the conversion of Suc into Glc and Fru, and the active uptake by a monosaccharide transporter into the pollen tube is presented.

1   This work was supported in part by a grant from the Spanish Ministry of Science and Education to D.G.
2   Present address: Plant Gene Expression Center, U.S. Department of Agriculture-Agricultural Research Station, University of California-Berkeley, 800 Buchanan Street, Albany, CA 94710.
3   These authors contributed equally to this work.
*   Corresponding author; e-mail A.J.vanTunen{at}cpro.dlo.nl; fax 31-317-418094.

Plant Physiol. (1998) 118: 297-304
Copyright Clearance Center:   0032-0889/98/118//08
© 1998 American Society of Plant Physiologists




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