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The Two Genes Encoding Starch-Branching Enzymes IIa and IIb Are
Differentially Expressed in Barley1
Chuanxin Sun,
Puthigae Sathish,
Staffan Ahlandsberg, and
Christer Jansson*
Department of Biochemistry, The Arrhenius Laboratories, Stockholm
University, S-106 91 Stockholm, Sweden
The sbeIIa and
sbeIIb genes, encoding starch-branching enzyme (SBE) IIa
and SBEIIb in barley (Hordeum vulgare L.), have been isolated. The 5 portions of the two genes are strongly divergent, primarily due to the 2064-nucleotide-long intron 2 in
sbeIIb. The sequence of this intron shows that it
contains a retro-transposon-like element. Expression of
sbeIIb but not sbeIIa was found to be
endosperm specific. The temporal expression patterns for
sbeIIa and sbeIIb were similar and peaked
around 12 d after pollination. DNA gel-blot analysis demonstrated
that sbeIIa and sbeIIb are both
single-copy genes in the barley genome. By fluorescence in situ
hybridization, the sbeIIa and sbeIIb
genes were mapped to chromosomes 2 and 5, respectively. The cDNA clones
for SBEIIa and SBEIIb were isolated and sequenced. The amino acid
sequences of SBEIIa and SBEIIb were almost 80% identical. The major
structural difference between the two enzymes was the presence of a
94-amino acid N-terminal extension in the SBEIIb precursor. The
( / )8-barrel topology of the -amylase superfamily
and the catalytic residues implicated in branching enzymes are
conserved in both barley enzymes.
1
This work was supported by the European Union
Biotechnology Program (no. FAIR-CT95-0568) and by the Foundation for
Strategic Research.
*
Corresponding author; e-mail christer{at}biokemi.su.se; fax
46-8-153679.
Plant Physiol. (1998) 118: 37-49
Copyright Clearance Center: 0032-0889/98/118//13
© 1998 American Society of Plant Physiologists
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