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Developmental Regulation of Intercellular Protein Trafficking through Plasmodesmata in Tobacco Leaf Epidermis1

Asuka Itaya, Young-Min Woo, Chikara Masuta, Yiming Bao, Richard S. Nelson, and Biao Ding*

Department of Botany, Oklahoma State University, Stillwater, Oklahoma 74078 (A.I., Y.-M.W., B.D.); Plant Virology Laboratory, Faculty of Agriculture, Hokkaido University, Sapporo 060, Japan (C.M.); and Plant Biology Division, The Samuel Roberts Noble Foundation, Ardmore, Oklahoma 73402 (Y.B., R.S.N.)

Plasmodesmata mediate direct cell-to-cell communication in plants. One of their significant features is that primary plasmodesmata formed at the time of cytokinesis often undergo structural modifications, by the de novo addition of cytoplasmic strands across cell walls, to become complex secondary plasmodesmata during plant development. Whether such modifications allow plasmodesmata to gain special transport functions has been an outstanding issue in plant biology. Here we present data showing that the cucumber mosaic virus 3a movement protein (MP):green fluorescent protein (GFP) fusion was not targeted to primary plasmodesmata in the epidermis of young or mature leaves in transgenic tobacco (Nicotiana tabacum) plants constitutively expressing the 3a:GFP fusion gene. Furthermore, the cucumber mosaic virus 3a MP:GFP fusion protein produced in planta by biolistic bombardment of the 3a:GFP fusion gene did not traffic between cells interconnected by primary plasmodesmata in the epidermis of a young leaf. In contrast, the 3a MP:GFP was targeted to complex secondary plasmodesmata and trafficked from cell to cell when a leaf reached a certain developmental stage. These data provide the first experimental evidence, to our knowledge, that primary and complex secondary plasmodesmata have different protein-trafficking functions and suggest that complex secondary plasmodesmata may be formed to traffic specific macromolecules that are important for certain stages of leaf development.

1   This study was supported by a grant from The Samuel Roberts Noble Foundation (to B.D. and R.S.N.), by a grant from the U.S. Department of Agriculture National Research Initiative Competitive Grants Program (no. 97-35303-4519 to B.D.), and by a Dean's Incentive Grant from the College of Arts and Sciences, Oklahoma State University (to B.D.).
*   Corresponding author; e-mail bxding{at}osuunx.ucc.okstate.edu;fax 1-405-744-7074.

Plant Physiol. (1998) 118: 373-385
Copyright Clearance Center:   0032-0889/98/118//13
© 1998 American Society of Plant Physiologists




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