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Metallothioneins 1 and 2 Have Distinct but Overlapping Expression
Patterns in Arabidopsis1
Margarita García-Hernández,
Angus Murphy, and
Lincoln Taiz*
Biology Department, Sinsheimer Laboratories, University of
California, Santa Cruz, California 95064
The spatial and temporal expression
patterns of metallothionein (MT) isoforms MT1a and
MT2a were investigated in vegetative and reproductive
tissues of untreated and copper-treated Arabidopsis by in situ
hybridization and by northern blotting. In control plants,
MT1a mRNA was localized in leaf trichomes and in the
vascular tissue in leaves, roots, flowers, and germinating embryos. In copper-treated plants, MT1a expression was also observed
in the leaf mesophyll and in vascular tissue of developing siliques and seeds. In contrast, MT2a was expressed primarily in the
trichomes of both untreated and copper-treated plants. In
copper-treated plants, MT2a mRNA was also expressed in
siliques. Northern-hybridization studies performed on developing
seedlings and leaves showed temporal variations of MT1a
gene expression but not of MT2a expression. The possible
implications of these findings for the cellular roles of MTs in plants
are discussed.
1
This study was supported by a grant from the
U.S. Department of Agriculture (no. 94-37100-0755) to L.T. and by a
Research Scientist Training Postdoctoral Fellowship from the Ministry
of Education and Sciences of Spain to M.G.-H.
*
Corresponding author; e-mail taiz{at}biology.ucsc.edu; fax
1-408-459-3139.
Plant Physiol. (1998) 118: 387-397
Copyright Clearance Center: 0032-0889/98/118//11
© 1998 American Society of Plant Physiologists
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