Plant Physiol. Journal of Pharmacology and Experimental Therapeutics
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Photosynthetic and Heterotrophic Ferredoxin Isoproteins Are Colocalized in Fruit Plastids of Tomato1

Koh Aoki2, Miyuki Yamamoto, and Keishiro Wada*

Department of Biology, Faculty of Science, Kanazawa University, Kakuma, Kanazawa 920-1192 Japan (K.A., K.W.); and Department of Anatomy 1, School of Medicine, Kanazawa University, 13-1 Takara-machi, Kanazawa 920-8640 Japan (M.Y.)

Fruit tissues of tomato (Lycopersicon esculentum Mill.) contain both photosynthetic and heterotrophic ferredoxin (FdA and FdE, respectively) isoproteins, irrespective of their photosynthetic competence, but we did not previously determine whether these proteins were colocalized in the same plastids. In isolated fruit chloroplasts and chromoplasts, both FdA and FdE were detected by immunoblotting. Colocalization of FdA and FdE in the same plastids was demonstrated using double-staining immunofluorescence microscopy. We also found that FdA and FdE were colocalized in fruit chloroplasts and chloroamyloplasts irrespective of sink status of the plastid. Immunoelectron microscopy demonstrated that FdA and FdE were randomly distributed within the plastid stroma. To investigate the significance of the heterotrophic Fd in fruit plastids, Glucose 6-phosphate dehydrogenase (G6PDH) activity was measured in isolated fruit and leaf plastids. Fruit chloroplasts and chromoplasts showed much higher G6PDH activity than did leaf chloroplasts, suggesting that high G6PDH activity is linked with FdE to maintain nonphotosynthetic production of reducing power. This result suggested that, despite their morphological resemblance, fruit chloroplasts are functionally different from their leaf counterparts.

1   This work was supported by research fellowships from the Japan Society for the Promotion of Science for Young Scientists to K.A. and by a Grant-in-Aid for Scientific Research from the Ministry of Education, Science, Culture, and Sports, Japan, to K.W. (no. 05454016).
2   Present address: Section of Plant Biology, Division of Biological Sciences, University of California, Davis, CA 95616-8537.
*   Corresponding author; e-mail keiwada{at}kenroku.kanazawa-u.ac.jp; fax 81-76-264-5745.

Plant Physiol. (1998) 118: 439-449
Copyright Clearance Center:   0032-0889/98/118//11
© 1998 American Society of Plant Physiologists




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