Plant Physiol. email content delivery
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via ISI Web of Science (29)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Schaller, H.
Right arrow Articles by Benveniste, P.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Schaller, H.
Right arrow Articles by Benveniste, P.
Agricola
Right arrow Articles by Schaller, H.
Right arrow Articles by Benveniste, P.

Overexpression of an Arabidopsis cDNA Encoding a Sterol-C241-Methyltransferase in Tobacco Modifies the Ratio of 24-Methyl Cholesterol to Sitosterol and Is Associated with Growth Reduction

Hubert Schaller*, Pierrette Bouvier-Navé, and Pierre Benveniste

Institut de Biologie Moléculaire des Plantes, Département d'Enzymologie Cellulaire et Moléculaire, Institut de Botanique, 28 rue Goethe, 67083 Strasbourg, France

Higher plants synthesize 24-methyl sterols and 24-ethyl sterols in defined proportions. As a first step in investigating the physiological function of this balance, an Arabidopsis cDNA encoding an S-adenosyl-L-methionine 24-methylene lophenol-C241-methyltransferase, the typical plant enzyme responsible for the production of 24-ethyl sterols, was expressed in tobacco (Nicotiana tabacum L.) under the control of a constitutive promoter. Transgenic plants displayed a novel 24-alkyl-Delta 5-sterol profile: the ratio of 24-methyl cholesterol to sitosterol, which is close to 1 in the wild type, decreased dramatically to values ranging from 0.01 to 0.31. In succeeding generations of transgenic tobacco, a high S-adenosyl-L-methionine 24-methylene lophenol-C241-methyltransferase enzyme activity and, consequently, a low ratio of 24-methyl cholesterol to sitosterol, was associated with reduced growth compared with the wild type. However, this new morphological phenotype appeared only below the threshold ratio of 24-methyl cholesterol to sitosterol of approximately 0.1. Because the size of cells was unchanged in small, transgenic plants, we hypothesize that a radical decrease of 24-methyl cholesterol and/or a concomitant increase of sitosterol would be responsible for a change in cell division through as-yet unknown mechanisms.

*   Corresponding author; e-mail schaller{at}medoc.u-strasbg.fr; fax 3-88-35-84-84.

Plant Physiol. (1998) 118: 461-469
Copyright Clearance Center:   0032-0889/98/118//09
© 1998 American Society of Plant Physiologists




This article has been cited by other articles:


Home page
 Plant Physiol.Home page
M. O'Brien, S.-C. Chantha, A. Rahier, and D. P. Matton
Lipid Signaling in Plants. Cloning and Expression Analysis of the Obtusifoliol 14{alpha}-Demethylase from Solanum chacoense Bitt., a Pollination- and Fertilization-Induced Gene with Both Obtusifoliol and Lanosterol Demethylase Activity
Plant Physiology, October 1, 2005; 139(2): 734 - 749.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
L. Arnqvist, P. C. Dutta, L. Jonsson, and F. Sitbon
Reduction of Cholesterol and Glycoalkaloid Levels in Transgenic Potato Plants by Overexpression of a Type 1 Sterol Methyltransferase cDNA
Plant Physiology, April 1, 2003; 131(4): 1792 - 1799.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
L. Didierjean, L. Gondet, R. Perkins, S.-M. C. Lau, H. Schaller, D. P. O'Keefe, and D. Werck-Reichhart
Engineering Herbicide Metabolism in Tobacco and Arabidopsis with CYP76B1, a Cytochrome P450 Enzyme from Jerusalem Artichoke
Plant Physiology, September 1, 2002; 130(1): 179 - 189.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
N. Holmberg, M. Harker, C. L. Gibbard, A. D. Wallace, J. C. Clayton, S. Rawlins, A. Hellyer, and R. Safford
Sterol C-24 Methyltransferase Type 1 Controls the Flux of Carbon into Sterol Biosynthesis in Tobacco Seed
Plant Physiology, September 1, 2002; 130(1): 303 - 311.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
D. R. Corbin, R. J. Grebenok, T. E. Ohnmeiss, J. T. Greenplate, and J. P. Purcell
Expression and Chloroplast Targeting of Cholesterol Oxidase in Transgenic Tobacco Plants
Plant Physiology, July 1, 2001; 126(3): 1116 - 1128.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
S. Munné-Bosch and L. Alegre
Subcellular Compartmentation of the Diterpene Carnosic Acid and Its Derivatives in the Leaves of Rosemary
Plant Physiology, February 1, 2001; 125(2): 1094 - 1102.
[Abstract] [Full Text]

Home page
 Plant CellHome page
A. C. Diener, H. Li, W.-x. Zhou, W. J. Whoriskey, W. D. Nes, and G. R. Fink
STEROL METHYLTRANSFERASE 1 Controls the Level of Cholesterol in Plants
PLANT CELL, June 1, 2000; 12(6): 853 - 870.
[Abstract] [Full Text]

Home page
 J. Biol. Chem.Home page
D. Warnecke, R. Erdmann, A. Fahl, B. Hube, F. Muller, T. Zank, U. Zahringer, and E. Heinz
Cloning and Functional Expression of UGT Genes Encoding Sterol Glucosyltransferases from Saccharomyces cerevisiae, Candida albicans, Pichia pastoris, and Dictyostelium discoideum
J. Biol. Chem., May 7, 1999; 274(19): 13048 - 13059.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
ASPB Publications PLANT PHYSIOLOGY THE PLANT CELL
Copyright © 1998 by the American Society of Plant Biologists