Plant Physiol.
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Changes in Growth CO2 Result in Rapid Adjustments of Ribulose-1,5-Bisphosphate Carboxylase/Oxygenase Small Subunit Gene Expression in Expanding and Mature Leaves of Rice1

Russ W. Gesch*, Kenneth J. Boote, Joseph C.V. Vu, L. Hartwell Allen Jr, and George Bowes

Department of Agronomy (R.W.G., K.J.B.), United States Department of Agriculture-Agricultural Research Service (J.C.V.V., L.H.A.), and Department of Botany (G.B.), University of Florida, Gainesville, Florida 32611

The accumulation of soluble carbohydrates resulting from growth under elevated CO2 may potentially signal the repression of gene activity for the small subunit of ribulose-1,5-bisphosphate carboxylase/oxygenase (rbcS). To test this hypothesis we grew rice (Oryza sativa L.) under ambient (350 µL L-1) and high (700 µL L-1) CO2 in outdoor, sunlit, environment-controlled chambers and performed a cross-switching of growth CO2 concentration at the late-vegetative phase. Within 24 h, plants switched to high CO2 showed a 15% and 23% decrease in rbcS mRNA, whereas plants switched to ambient CO2 increased 27% and 11% in expanding and mature leaves, respectively. Ribulose-1,5-bisphosphate carboxylase/oxygenase total activity and protein content 8 d after the switch increased up to 27% and 20%, respectively, in plants switched to ambient CO2, but changed very little in plants switched to high CO2. Plants maintained at high CO2 showed greater carbohydrate pool sizes and lower rbcS transcript levels than plants kept at ambient CO2. However, after switching growth CO2 concentration, there was not a simple correlation between carbohydrate and rbcS transcript levels. We conclude that although carbohydrates may be important in the regulation of rbcS expression, changes in total pool size alone could not predict the rapid changes in expression that we observed.

1   This work was supported by the U.S. Department of Agriculture/National Research Initiative Competitive Grants Program Plant Responses to the Environment (grant no. 95-37100-1597). This is Florida Agricultural Experiment Station journal series no. R-06325.
*   Corresponding author; e-mail rwg30{at}nervm.nerdc.ufl.edu; fax 1-352-392-6139.

Plant Physiol. (1998) 118: 521-529
Copyright Clearance Center:   0032-0889/98/118//09
© 1998 American Society of Plant Physiologists




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