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Influence of Water Content and Temperature on Molecular Mobility
and Intracellular Glasses in Seeds and Pollen1
Julia Buitink*,
Mireille M.A.E. Claessens,
Marcus A. Hemminga, and
Folkert A. Hoekstra
Wageningen Agricultural University, Laboratory of Plant Physiology,
Arboretumlaan 4, 6703 BD Wageningen, The Netherlands (J.B., F.A.H.,
M.M.A.E.C.); and Laboratory of Molecular Physics, Dreijenlaan 3, 6703 HA Wageningen, The Netherlands (J.B., M.M.A.E.C., M.A.H.)
Although the occurrence of
intracellular glasses in seeds and pollen has been established,
physical properties such as rotational correlation times and viscosity
have not been studied extensively. Using electron paramagnetic
resonance spectroscopy, we examined changes in the molecular mobility
of the hydrophilic nitroxide spin probe 3-carboxy-proxyl during melting
of intracellular glasses in axes of pea (Pisum sativum
L.) seeds and cattail (Typha latifolia L.) pollen. The
rotational correlation time of the spin probe in intracellular glasses
of both organisms was approximately 10 3 s. Using the
distance between the outer extrema of the electron paramagnetic
resonance spectrum (2Azz) as a measure of molecular mobility, we found a sharp increase in mobility at a definite temperature during heating. This temperature increased with decreasing water content of the samples. Differential scanning calorimetry data on
these samples indicated that this sharp increase corresponded to
melting of the glassy matrix. Molecular mobility was found to be
inversely correlated with storage stability. With decreasing water
content, the molecular mobility reached a minimum, and increased again
at very low water content. Minimum mobility and maximum storage
stability occurred at a similar water content. This correlation suggests that storage stability might be at least partially controlled by molecular mobility. At low temperatures, when storage longevity cannot be determined on a realistic time scale, 2Azz
measurements can provide an estimate of the optimum storage conditions.
1
This research was financially supported by the
Netherlands Technology Foundation (STW) and was coordinated by the Life
Sciences Foundation.
*
Corresponding author; e-mail julia.buitink{at}algem.pf.wau.nl; fax
31-317-484740.
Plant Physiol. (1998) 118: 531-541
Copyright Clearance Center: 0032-0889/98/118//11
© 1998 American Society of Plant Physiologists
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