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Two Genetically Separable Phases of Growth Inhibition Induced by Blue Light in Arabidopsis Seedlings1

Brian M. Parks, Myeon H. Cho2, and Edgar P. Spalding*

Department of Botany, University of Wisconsin, 430 Lincoln Drive, Madison, Wisconsin 53706

High fluence-rate blue light (BL) rapidly inhibits hypocotyl growth in Arabidopsis, as in other species, after a lag time of 30 s. This growth inhibition is always preceded by the activation of anion channels. The membrane depolarization that results from the activation of anion channels by BL was only 30% of the wild-type magnitude in hy4, a mutant lacking the HY4 BL receptor. High-resolution measurements of growth made with a computer-linked displacement transducer or digitized images revealed that BL caused a rapid inhibition of growth in wild-type and hy4 seedlings. This inhibition persisted in wild-type seedlings during more than 40 h of continuous BL. By contrast, hy4 escaped from the initial inhibition after approximately 1 h of BL and grew faster than wild type for approximately 30 h. Wild-type seedlings treated with 5-nitro-2-(3-phenylpropylamino)-benzoic acid, a potent blocker of the BL-activated anion channel, displayed rapid growth inhibition, but, similar to hy4, these seedlings escaped from inhibition after approximately 1 h of BL and phenocopied the mutant for at least 2.5 h. The effects of 5-nitro-2-(3-phenylpropylamino)-benzoic acid and the HY4 mutation were not additive. Taken together, the results indicate that BL acts through HY4 to activate anion channels at the plasma membrane, causing growth inhibition that begins after approximately 1 h. Neither HY4 nor anion channels appear to participate greatly in the initial phase of inhibition.

1   This work was supported by the National Aeronautics and Space Administration/National Science Foundation Network for Research on Plant Sensory Systems (grant no. IBN-9416016) and a grant to the University of Wisconsin from the Department of Energy/National Science Foundation/U.S. Department of Agriculture Collaborative Program on Research in Plant Biology (grant no. BIR 92-20331).
2   Present address: Department of Biology, Yonsei University, 134 Sinchon-Dong, Seoul, 120-749, Korea.
*   Corresponding author; e-mail spalding{at}facstaff.wisc.edu; fax 1-608-262-7509.

Plant Physiol. (1998) 118: 609-615
Copyright Clearance Center:   0032-0889/98/118//07
© 1998 American Society of Plant Physiologists




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