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Carbohydrate and Amino Acid Metabolism in the Eucalyptus globulus-Pisolithus tinctorius Ectomycorrhiza during Glucose Utilization1

Francis Martin*, Vincent Boiffin, and Philip E. Pfeffer

Equipe de Microbiologie Forestière, Institut National de la Recherche Agronomique, Centre de Recherches de Nancy, F-54280 Champenoux, France (F.M., V.B.); and Plant-Soil Biophysics, United States Department of Agriculture-Agricultural Research Service, 600 East Mermaid Lane, Wyndmoor, Pennsylvania 19038 (P.E.P.)

The metabolism of [1-13C]glucose in Pisolithus tinctorius cv Coker & Couch, in uninoculated seedlings of Eucalyptus globulus bicostata ex Maiden cv Kirkp., and in the E. globulus-P. tinctorius ectomycorrhiza was studied using nuclear magnetic resonance spectroscopy. In roots of uninoculated seedlings, the 13C label was mainly incorporated into sucrose and glutamine. The ratio (13C3 + 13C2)/13C4 of glutamine was approximately 1.0 during the time-course experiment, indicating equivalent contributions of phosphoenolpyruvate carboxylase and pyruvate dehydrogenase to the production of alpha -ketoglutarate used for synthesis of this amino acid. In free-living P. tinctorius, most of the 13C label was incorporated into mannitol, trehalose, glutamine, and alanine, whereas arabitol, erythritol, and glutamate were weakly labeled. Amino acid biosynthesis was an important sink of assimilated 13C (43%), and anaplerotic CO2 fixation contributed 42% of the C flux entering the Krebs cycle. In ectomycorrhizae, sucrose accumulation was decreased in the colonized roots compared with uninoculated control plants, whereas 13C incorporation into arabitol and erythritol was nearly 4-fold higher in the symbiotic mycelium than in the free-living fungus. It appears that fungal utilization of glucose in the symbiotic state is altered and oriented toward the synthesis of short-chain polyols.

1   This work was supported by a travel grant from the Institut National de la Recherche Agronomique (to F.M.). V.B. was supported by a doctoral scholarship from the Ministère de l'Enseignement Supérieur et de la Recherche.
*   Corresponding author; e-mail fmartin{at}nancy.inra.fr; fax 33-383-394069.

Plant Physiol. (1998) 118: 627-635
Copyright Clearance Center:   0032-0889/98/118//09
© 1998 American Society of Plant Physiologists




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