Plant Physiol.
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via CrossRef
Right arrow Citing Articles via Web of Science (30)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Fujita, T.
Right arrow Articles by Csonka, L. N.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Fujita, T.
Right arrow Articles by Csonka, L. N.
Agricola
Right arrow Articles by Fujita, T.
Right arrow Articles by Csonka, L. N.

Comparative Analysis of the Regulation of Expression and Structures of Two Evolutionarily Divergent Genes for Delta 1-Pyrroline-5-Carboxylate Synthetase from Tomato1

Tomomichi Fujita, Albino Maggio, Mario Garcia-Rios2, Ray A. Bressan, and Laszlo N. Csonka*

Departments of Biological Sciences (T.F., M.G.-R., L.N.C.) and Horticulture (A.M., R.A.B.), Purdue University, West Lafayette, Indiana 47907

We isolated two tomato (Lycopersicon esculentum) cDNA clones, tomPRO1 and tomPRO2, specifying Delta 1-pyrroline-5-carboxylate synthetase (P5CS), the first enzyme of proline (Pro) biosynthesis. tomPRO1 is unusual because it resembles prokaryotic polycistronic operons (M.G. García-Ríos, T. Fujita, P.C. LaRosa, R.D. Locy, J.M. Clithero, R.A. Bressan, L.N. Csonka [1997] Proc Natl Acad Sci USA 94: 8249-8254), whereas tomPRO2 encodes a full-length P5CS. We analyzed the accumulation of Pro and the tomPRO1 and tomPRO2 messages in response to NaCl stress and developmental signals. Treatment with 200 mM NaCl resulted in a >60-fold increase in Pro levels in roots and leaves. However, there was a <3-fold increase in the accumulation of the tomPRO2 message and no detectable induction in the level of the tomPRO1 message in response to NaCl stress. Although pollen contained approximately 100-fold higher levels of Pro than other plant tissues, there was no detectable increase in the level of either message in pollen. We conclude that transcriptional regulation of these genes for P5CS is probably not important for the osmotic or pollen-specific regulation of Pro synthesis in tomato. Using restriction fragment-length polymorphism mapping, we determined the locations of tomPRO1 and tomPRO2 loci in the tomato nuclear genome. Sequence comparison suggested that tomPRO1 is similar to prokaryotic P5CS loci, whereas tomPRO2 is closely related to other eukaryotic P5CS genes.

1   This work was funded by the U.S. Department of Agriculture (grant no. 93-37100-8871).
2   Present address: Department of Natural Sciences, Texas A&M International University, Laredo, TX 78041.
*   Corresponding author; e-mail lcsonka{at}bilbo.bio.purdue.edu; fax 1-765-496-1496.

Plant Physiol. (1998) 118: 661-674
Copyright Clearance Center:   0032-0889/98/118//14
© 1998 American Society of Plant Physiologists




This article has been cited by other articles:


Home page
 Appl. Environ. Microbiol.Home page
T. Sekine, A. Kawaguchi, Y. Hamano, and H. Takagi
Desensitization of Feedback Inhibition of the Saccharomyces cerevisiae {gamma}-Glutamyl Kinase Enhances Proline Accumulation and Freezing Tolerance
Appl. Envir. Microbiol., June 15, 2007; 73(12): 4011 - 4019.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
S. Grallath, T. Weimar, A. Meyer, C. Gumy, M. Suter-Grotemeyer, J.-M. Neuhaus, and D. Rentsch
The AtProT Family. Compatible Solute Transporters with Similar Substrate Specificity But Differential Expression Patterns
Plant Physiology, January 1, 2005; 137(1): 117 - 126.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
T. Fujita, A. Maggio, M. Garcia-Rios, C. Stauffacher, R. A. Bressan, and L. N. Csonka
Identification of Regions of the Tomato gamma -Glutamyl Kinase That Are Involved in Allosteric Regulation by Proline
J. Biol. Chem., April 11, 2003; 278(16): 14203 - 14210.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
N. H. Roosens, R. Willem, Y. Li, I. Verbruggen, M. Biesemans, and M. Jacobs
Proline Metabolism in the Wild-Type and in a Salt-Tolerant Mutant of Nicotiana plumbaginifolia Studied by 13C-Nuclear Magnetic Resonance Imaging
Plant Physiology, December 1, 1999; 121(4): 1281 - 1290.
[Abstract] [Full Text]

Home page
 Plant Physiol.Home page
A. P. Stines, D. J. Naylor, P. B. Høj, and R. van Heeswijck
Proline Accumulation in Developing Grapevine Fruit Occurs Independently of Changes in the Levels of Delta 1-Pyrroline-5-Carboxylate Synthetase mRNA or Protein
Plant Physiology, July 1, 1999; 120(3): 923 - 923.
[Abstract] [Full Text]

Home page
 Plant CellHome page
R. Schwacke, S. Grallath, K. E. Breitkreuz, E. Stransky, H. Stransky, W. B. Frommer, and D. Rentsch
LeProT1, a Transporter for Proline, Glycine Betaine, and {gamma}-Amino Butyric Acid in Tomato Pollen
PLANT CELL, March 1, 1999; 11(3): 377 - 392.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
ASPB Publications PLANT PHYSIOLOGY® THE PLANT CELL
Copyright © 1998 by the American Society of Plant Biologists