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Differential Expression of Alternative Oxidase Genes in Soybean
Cotyledons during Postgerminative Development1
Tulene C. McCabe,
Patrick M. Finnegan,
A. Harvey
Millar2,
David A. Day, and
James Whelan*
Department of Biochemistry, The University of Western Australia,
Nedlands, Perth, WA 6907, Australia (T.C.M., J.W.); and Division of
Biochemistry and Molecular Biology, Faculty of Science, The Australian
National University, Canberra, ACT 0200, Australia (P.M.F., A.H.M.,
D.A.D.)
The
expression of the alternative oxidase (AOX) was investigated during
cotyledon development in soybean (Glycine max [L.] Merr.) seedlings. The total amount of AOX protein increased throughout development, not just in earlier stages as previously thought, and was
correlated with the increase in capacity of the alternative pathway.
Each AOX isoform (AOX1, AOX2, and AOX3) showed a different developmental trend in mRNA abundance, such that the increase in AOX
protein and capacity appears to involve a shift in gene expression from
AOX2 to AOX3. As the cotyledons aged, the
size of the mitochondrial ubiquinone pool decreased. We discuss how this and other factors may affect the alternative pathway activity that
results from the developmental regulation of AOX expression.
1
This work was supported by Australian Research
Council grants to J.W. and D.A.D. and by an Australian postgraduate
award from the Australian Commonwealth Government to
T.C.M.
2
Present address: Department of Plant Sciences,
University of Oxford, Oxford OX1 3RB, UK.
*
Corresponding author; e-mail seamus{at}cyllene.uwa.edu.au; fax
61-8-9380-1148.
Plant Physiol. (1998) 118: 675-682
Copyright Clearance Center: 0032-0889/98/118//08
© 1998 American Society of Plant Physiologists
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