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The Heat-Shock Element Is a Functional Component of the Arabidopsis APX1 Gene Promoter1

Sergei Storozhenko, Pascal De Pauw, Marc Van Montagu*, Dirk Inzé, and Sergei Kushnir

Laboratorium voor Genetica, Departement Genetica, Vlaams Interuniversitair Instituut voor Biotechnologie (S.S., P.D.P., M.V.M., S.K.); and Laboratoire Associé de l'Institut National de la Recherche Agronomique (France) (D.I.), Universiteit Gent, K.L. Ledeganckstraat 35, B-9000 Gent, Belgium

Ascorbate peroxidases are important enzymes that detoxify hydrogen peroxide within the cytosol and chloroplasts of plant cells. To better understand their role in oxidative stress tolerance, the transcriptional regulation of the apx1 gene from Arabidopsis was studied. The apx1 gene was expressed in all tested organs of Arabidopsis; mRNA levels were low in roots, leaves, and stems and high in flowers. Steady-state mRNA levels in leaves or cell suspensions increased after treatment with methyl viologen, ethephon, high temperature, and illumination of etiolated seedlings. A putative heat-shock cis element found in the apx1 promoter was shown to be recognized by the tomato (Lycopersicon esculentum) heat-shock factor in vitro and to be responsible for the in vivo heat-shock induction of the gene. The heat-shock cis element also contributed partially to the induction of the gene by oxidative stress. By using in vivo dimethyl sulfate footprinting, we showed that proteins interacted with a G/C-rich element found in the apx1 promoter.

1   This research was supported by grants from the Belgian Program on Interuniversity Poles of Attraction (Prime Minister's Office, Science Policy Programming, no. 38) and the Vlaams Actieprogramma Biotechnologie (no. ETC 002), by the International Human Frontier Science Program (no. RG-43494M), and by the European Community Biotech Program as part of the Project of Technological Priority (1993-1996). D.I. is a Research Director of the Institut National de la Recherche Agronomique (France).
*   Corresponding author; e-mail mamon{at}gengenp.rug.ac.be; fax 32-9-264-5349.

Plant Physiol. (1998) 118: 1005-1014
Copyright Clearance Center:   0032-0889/98/118//10
© 1998 American Society of Plant Physiologists




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