Plant Physiol. Illumina
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via CrossRef
Right arrow Citing Articles via Web of Science (40)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Lush, W. M.
Right arrow Articles by Wolters-Arts, M.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Lush, W. M.
Right arrow Articles by Wolters-Arts, M.
Agricola
Right arrow Articles by Lush, W. M.
Right arrow Articles by Wolters-Arts, M.

Directional Guidance of Nicotiana alata Pollen Tubes in Vitro and on the Stigma1

W. Mary Lush*, Franz Grieser, and Mieke Wolters-Arts

Plant Cell Biology Research Centre, School of Botany (W.M.L.), and School of Chemistry (F.G.), University of Melbourne, Parkville, Victoria 3052, Australia; and University of Melbourne, Parkville, Victoria 3052, AustraliaDepartment of Experimental Botany, University of Nijmegen, Toernooiveld 1, 6525 Nijmegen, The Netherlands (M.W.-A.)

Pollen tubes navigate the route from stigma to ovule with great accuracy, but the cues that guide them along this route are not known. We reproduced the environment on the stigma of Nicotiana alata by immersing pollen in stigma exudate or oil close to an interface with an aqueous medium. The growth of pollen in this culture system mimicked growth on stigmas: pollen grains hydrated and germinated, and pollen tubes grew toward the aqueous medium. The rate-limiting step in pollen germination was the movement of water through the surrounding exudate or oil. By elimination of other potential guidance cues, we conclude that the directional supply of water probably determined the axis of polarity of pollen tubes and resulted in growth toward the interface. We propose that a gradient of water in exudate is a guidance cue for pollen tubes on the stigma and that the composition of the exudate must be such that it is permeable enough for pollen hydration to occur but not so permeable that the supply of water becomes nondirectional. Pollen tube penetration of the stigma may be the most frequently occurring hydrotropic response of higher plants.

1   This work was supported by a special research grant from the Australian Research Council to the Plant Cell Biology Research Centre.
*   Corresponding author; e-mail w.lush{at}botany.unimelb.edu.au; fax 61-3-9347-1071.

Plant Physiol. (1998) 118: 733-741
Copyright Clearance Center:   0032-0889/98/118//09
© 1998 American Society of Plant Physiologists




This article has been cited by other articles:


Home page
 Plant Physiol.Home page
T. S. Feild, D. S. Chatelet, and T. J. Brodribb
Giant Flowers of Southern Magnolia Are Hydrated by the Xylem
Plant Physiology, July 1, 2009; 150(3): 1587 - 1597.
[Abstract] [Full Text] [PDF]

Home page
 GeneticsHome page
L. C. Boavida, B. Shuai, H.-J. Yu, G. C. Pagnussat, V. Sundaresan, and S. McCormick
A Collection of Ds Insertional Mutants Associated With Defects in Male Gametophyte Development and Function in Arabidopsis thaliana
Genetics, April 1, 2009; 181(4): 1369 - 1385.
[Abstract] [Full Text] [PDF]

Home page
 Mol PlantHome page
A. M. Prado, R. Colaco, N. Moreno, A. C. Silva, and J. A. Feijo
Targeting of Pollen Tubes to Ovules Is Dependent on Nitric Oxide (NO) Signaling
Mol Plant, July 1, 2008; 1(4): 703 - 714.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Bot.Home page
J. Lyew, Z. Li, Y. Liang-Chen, L. Yi-bo, and T. L. Sage
Pollen tube growth in association with a dry-type stigmatic transmitting tissue and extragynoecial compitum in the basal angiosperm Kadsura longipedunculata (Schisandraceae)
Am. J. Botany, July 1, 2007; 94(7): 1170 - 1182.
[Abstract] [Full Text] [PDF]

Home page
 ANN BOT (LOND)Home page
K. HRISTOVA, M. LAM, T. FEILD, and T. L. SAGE
Transmitting Tissue ECM Distribution and Composition, and Pollen Germinability in Sarcandra glabra and Chloranthus japonicus (Chloranthaceae)
Ann. Bot., October 1, 2005; 96(5): 779 - 791.
[Abstract] [Full Text] [PDF]

Home page
 ANN BOT (LOND)Home page
G. BOHNE, H. WOEHLECKE, and R. EHWALD
Water Relations of the Pine Exine
Ann. Bot., August 1, 2005; 96(2): 201 - 208.
[Abstract] [Full Text] [PDF]

Home page
 GeneticsHome page
M. A. Johnson, K. von Besser, Q. Zhou, E. Smith, G. Aux, D. Patton, J. Z. Levin, and D. Preuss
Arabidopsis hapless Mutations Define Essential Gametophytic Functions
Genetics, October 1, 2004; 168(2): 971 - 982.
[Abstract] [Full Text] [PDF]

Home page
 DevelopmentHome page
A. M. Prado, D. M. Porterfield, and J. A. Feijo
Nitric oxide is involved in growth regulation and re-orientation of pollen tubes
Development, June 1, 2004; 131(11): 2707 - 2714.
[Abstract] [Full Text] [PDF]

Home page
 Plant CellHome page
A. F. Edlund, R. Swanson, and D. Preuss
Pollen and Stigma Structure and Function: The Role of Diversity in Pollination
PLANT CELL, June 1, 2004; 16(suppl_1): S84 - S97.
[Full Text] [PDF]

Home page
 ANN BOT (LOND)Home page
L. M. STONE, K. A. SEATON, J. KUO, and J. A. McCOMB
Fast Pollen Tube Growth in Conospermum Species
Ann. Bot., April 1, 2004; 93(4): 369 - 378.
[Abstract] [Full Text] [PDF]

Home page
 Plant Cell PhysiolHome page
M. Iwano, T. Entani, H. Shiba, S. Takayama, and A. Isogai
Calcium Crystals in the Anther of Petunia: the Existence and Biological Significance in the Pollination Process
Plant Cell Physiol., January 15, 2004; 45(1): 40 - 47.
[Abstract] [Full Text] [PDF]

Home page
 Mol Biol EvolHome page
N. Takebayashi, P. B. Brewer, E. Newbigin, and M. K. Uyenoyama
Patterns of Variation Within Self-Incompatibility Loci
Mol. Biol. Evol., November 1, 2003; 20(11): 1778 - 1794.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Bot.Home page
J. Sanzol, P. Rallo, and M. Herrero
Asynchronous development of stigmatic receptivity in the pear (Pyrus communis; Rosaceae) flower
Am. J. Botany, January 1, 2003; 90(1): 78 - 84.
[Abstract] [Full Text] [PDF]

Home page
 J Exp BotHome page
I. Barinova, M. Zhexembekova, E. Barsova, S. Lukyanov, E. Heberle-Bors, and A. Touraev
Antirrhinum majus microspore maturation and transient transformation in vitro
J. Exp. Bot., May 1, 2002; 53(371): 1119 - 1129.
[Abstract] [Full Text] [PDF]

Home page
 ScienceHome page
T. Higashiyama, S. Yabe, N. Sasaki, Y. Nishimura, S.-y. Miyagishima, H. Kuroiwa, and T. Kuroiwa
Pollen Tube Attraction by the Synergid Cell
Science, August 24, 2001; 293(5534): 1480 - 1483.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
S. A. Johnson and S. McCormick
Pollen Germinates Precociously in the Anthers of raring-to-go, an Arabidopsis Gametophytic Mutant
Plant Physiology, June 1, 2001; 126(2): 685 - 695.
[Abstract] [Full Text] [PDF]

Home page
 GeneticsHome page
N. Bechtold, B. Jaudeau, S. Jolivet, B. Maba, D. Vezon, R. Voisin, and G. Pelletier
The Maternal Chromosome Set Is the Target of the T-DNA in the in Planta Transformation of Arabidopsis thaliana
Genetics, August 1, 2000; 155(4): 1875 - 1887.
[Abstract] [Full Text]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
ASPB Publications PLANT PHYSIOLOGY® THE PLANT CELL
Copyright © 1998 by the American Society of Plant Biologists