Overexpression of 20-Oxidase Confers a Gibberellin-Overproduction Phenotype in Arabidopsis

Shihshieh Huang^*, Anuradha S. Raman, Joel E. Ream, Hideji Fujiwara, R. Eric Cerny, and Sherri M. Brown

Plant Growth and Development Group, Monsanto Company, 700 Chesterfield Parkway North, St. Louis, Missouri 63198

In the gibberellin (GA) biosynthesis pathway, 20-oxidase catalyzes the oxidation and elimination of carbon-20 to give rise to C₁₉-GAs. All bioactive GAs are C₁₉-GAs. We have overexpressed a cDNA encoding 20-oxidase isolated from Arabidopsis seedlings in transgenic Arabidopsis plants. These transgenic plants display a phenotype that may be attributed to the overproduction of GA. The phenotype includes a longer hypocotyl, lighter-green leaves, increased stem elongation, earlier flowering, and decreased seed dormancy. However, the fertility of the transgenic plants is not affected. Increased levels of endogenous GA₁, GA₉, and GA₂₀ were detected in seedlings of the transgenic line examined. GA₄, which is thought to be the predominantly active GA in Arabidopsis, was not present at increased levels in this line. These results suggest that the overexpression of this 20-oxidase increases the levels of some endogenous GAs in transgenic seedlings, which causes the GA-overproduction phenotype.

Plant Physiol. (1998) 118: 773-781
Copyright Clearance Center:   0032-0889/98/118//09
© 1998 American Society of Plant Physiologists

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