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Altered Zn Compartmentation in the Root Symplasm and Stimulated Zn Absorption into the Leaf as Mechanisms Involved in Zn Hyperaccumulation in Thlaspi caerulescens

Mitch M. Lasat, Alan J.M. Baker, and Leon V. Kochian*

United States Plant, Soil, and Nutrition Laboratory, United States Department of Agriculture-Agricultural Research Station, Cornell University, Ithaca, New York 14853 (M.M.L., L.V.K.); and Department of Animal and Plant Sciences, University of Sheffield, Sheffield, United Kingdom S10 2TN (A.J.M.B.)

We investigated Zn compartmentation in the root, Zn transport into the xylem, and Zn absorption into leaf cells in Thlaspi caerulescens, a Zn-hyperaccumulator species, and compared them with those of a related nonaccumulator species, Thlaspi arvense. Zn-compartmental analysis conducted with roots of the two species indicated that a significant fraction of symplasmic Zn was stored in the root vacuole of T. arvense, and presumably became unavailable for loading into the xylem and subsequent translocation to the shoot. In T. caerulescens, however, a smaller fraction of the absorbed Zn was stored in the root vacuole and was readily transported back into the cytoplasm. We conclude that in T. caerulescens, Zn absorbed by roots is readily available for loading into the xylem. This is supported by analysis of xylem exudate collected from detopped Thlaspi species seedlings. When seedlings of the two species were grown on either low (1 µM) or high (50 µM) Zn, xylem sap of T. caerulescens contained approximately 5-fold more Zn than that of T. arvense. This increase was not correlated with a stimulated production of any particular organic or amino acid. The capacity of Thlaspi species cells to absorb 65Zn was studied in leaf sections and leaf protoplasts. At low external Zn levels (10 and 100 µM), there was no difference in leaf Zn uptake between the two Thlaspi species. However, at 1 mM Zn2+, 2.2-fold more Zn accumulated in leaf sections of T. caerulescens. These findings indicate that altered tonoplast Zn transport in root cells and stimulated Zn uptake in leaf cells play a role in the dramatic Zn hyperaccumulation expressed in T. caerulescens.

*   Corresponding author; e-mail lvk1{at}cornell.edu; fax 1-607-255-1132.

Plant Physiol. (1998) 118: 875-883
Copyright Clearance Center:   0032-0889/98/118//09
© 1998 American Society of Plant Physiologists




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