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Abscisic Acid-Dependent and -Independent Expression of the Carrot
Late-Embryogenesis-Abundant-Class Gene Dc3 in Transgenic
Tobacco Seedlings1
Najeeb U. Siddiqui2,
Hwa-Jee Chung3,
Terry
L. Thomas, and
Malcolm C. Drew*
Department of Horticultural Sciences (N.U.S., M.C.D.), and
Department of Biology (H.-J.C., T.L.T.), Texas A&M University,
College Station, Texas 77843
We
studied the expression of three promoter 5 deletion constructs ( 218,
599, and 1312) of the LEA (late
embryogenesis abundant)-class gene
Dc3 fused to  -glucuronidase (GUS), where each
construct value refers to the number of base pairs upstream of the
transcription start site at which the deletion occurred. The
Dc3 gene is noted for its induction by abscisic acid
(ABA), but its response to other plant hormones and various
environmental stresses has not been reported previously for vegetative
cells. Fourteen-day-old transgenic tobacco (Nicotiana
tabacum L.) seedlings were exposed to dehydration, hypoxia,
salinity, exogenous ethylene, or exogenous methyl jasmonate (MeJa). GUS
activity was quantified fluorimetrically and expression was observed by
histochemical staining of the seedlings. An increase in GUS activity
was observed in plants with constructs 599 and 1312 in response to
dehydration and salinity within 6 h of stress, and at 12 h in
response to hypoxia. No increase in endogenous ABA was found in any of
the three lines, even after 72 h of hypoxia. An ABA-independent
increase in GUS activity was observed when endogenous ABA biosynthesis was blocked by fluridone and plants were exposed to 5 µL
L 1 ethylene in air or 100 µM MeJa.
Virtually no expression was observed in construct 218 in response to
dehydration, salinity, or MeJa, but there was a moderate response to
ethylene and hypoxia. This suggests that the region between 218 and
599 is necessary for ABA (dehydration and salinity)- and
MeJa-dependent expression, whereas ethylene-mediated expression does
not require this region of the promoter.
1
This work was supported by the U.S.
Department of Agriculture National Research Initiative Competitive
Grants Program (no. 9437304 to T.L.T.).
2
Present address: Department of Botany,
University of Toronto, Toronto, Ontario, Canada M5S 3B2.
3
Present address: Department of Horticultural
Science, University of Florida, Gainesville, FL 32611.
*
Corresponding author; e-mail mcdrew{at}tamu.edu; fax
1-409-845-0627.
Plant Physiol. (1998) 118: 1181-1190
Copyright Clearance Center: 0032-0889/98/118//10
© 1998 American Society of Plant Physiologists
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