Plant Physiol. Journal of Pharmacology and Experimental Therapeutics
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Developmental and Environmental Effects on the Expression of the C3-C4 Intermediate Phenotype in Moricandia arvensis1

Elizabeth L. Rylott2, Karin Metzlaff, and Stephen Rawsthorne*

John Innes Centre, Norwich Research Park, Colney, Norwich NR4 7UH, United Kingdom

Cellular anatomy and expression of glycine decarboxylase (GDC) protein were studied during leaf development of the C3-C4 intermediate species Moricandia arvensis. Leaf anatomy was initially C3-like and the number and profile area of mitochondria in the bundle-sheath cells were the same as those in adjacent mesophyll cells. Between a leaf length of 6 and 12 mm there was a bundle-sheath-specific, 4-fold increase in the number of mitochondrial profiles, followed by a doubling of their individual profile areas as the leaves expanded further. Subunits of GDC were present in whole-leaf extracts before the anatomical development of bundle-sheath cells. Whereas the GDC H-protein content of leaves increased steadily throughout development, the increase in GDC P-protein was synchronous with the development of mitochondria in the bundle sheath. The P-protein was confined to bundle-sheath mitochondria throughout leaf development, and its content in individual mitochondria increased before the anatomical development of the bundle sheath. Anatomical and biochemical attributes of the C3-C4 character were present in the cotyledons and sepals but not in other photosynthetic organs/tissues. In leaves and cotyledons that developed in the dark, the expression of the P-protein and the organellar development were reduced but the bundle-sheath cell specificity was retained.

1   This work was supported by a Competitive Strategic Grant to the John Innes Centre from the Biotechnology and Biological Sciences Research Council and by research grant no. PL962002 from the European Union Biotechnology Program. E.L.R. was supported by a Ph.D. studentship from the Gatsby Charitable Foundation.
2   Present address: Institute of Biomedical and Life Sciences, Division of Biochemistry and Molecular Biology, University of Glasgow, Glasgow G12 8QQ, UK.
*   Corresponding author; e-mail steve.rawsthorne{at}bbsrc.ac.uk; fax 44-1603-259882/456844.

Plant Physiol. (1998) 118: 1277-1284
Copyright Clearance Center:   0032-0889/98/118//08
© 1998 American Society of Plant Physiologists




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[Abstract] [Full Text] [PDF]


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