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Differential Regulation of Enolase during
Anaerobiosis in
Maize1
Shailesh K. Lal2,
Chwenfang Lee, and
Martin M. Sachs*
Department of Crop Sciences, University of Illinois at
Urbana-Champaign, Urbana, Illinois 61801 (S.K.L., C.L., M.M.S.); and United States Department of Agriculture/Agricultural Research Service,
Plant Physiology and Genetics Research Unit, Urbana, Illinois 61801 (M.M.S.)
It was
reported previously that enolase enzyme activity and ENO1 transcript
levels are induced by anaerobic stress in maize (Zea
mays). Here we show that not all isoforms of maize enolase are
anaerobically induced. We cloned and sequenced a second enolase cDNA
clone (pENO2) from maize. Sequence analysis showed that pENO2 shares
75.6% nucleotide and 89.5% deduced amino acid sequence identity with
pENO1 and is encoded by a distinct gene. Expression of ENO2 is
constitutive under aerobic conditions, whereas ENO1 levels are induced
10-fold in maize roots after 24 h of anaerobic treatment.
Western-blot analysis and N-terminal sequencing of in vivo-labeled
maize roots identified two major proteins selectively synthesized upon
anaerobic stress as isozymes of enolase. We describe the expression of
enolase in maize roots under anaerobic stress.
1
This work was supported by a grant from the U.S.
Public Health Service (National Institutes of Health no. 5 R01
GM34740), and by U.S. Department of Agriculture/Agricultural Research
Service funds awarded to M.M.S.
2
Present address: Program in Plant Molecular and
Cellular Biology and Horticultural Sciences, University of Florida,
Gainesville, FL 32611-0690.
*
Corresponding author; e-mail msachs{at}uiuc.edu; fax
1-217-333-6064.
Plant Physiol. (1998) 118: 1285-1293
Copyright Clearance Center: 0032-0889/98/118//09
© 1998 American Society of Plant Physiologists
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