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Comparison of Binding Properties and Early Biological Effects of
Elicitins in Tobacco Cells1
Stéphane Bourque,
Michel Ponchet,
Marie-Noëlle Binet,
Pierre Ricci,
Alain Pugin, and
Angela Lebrun-Garcia*
Unité Associée Institut National de la Recherche
Agronomique-Université de Bourgogne no. 692, Laboratoire de
Phytopharmacie et Biochimie des Interactions Cellulaires, BV 1540, 21 034 Dijon cedex, France (S.B., M.-N.B., A.P., A.L.-G.); and Station de
Botanique et de Pathologie Végétale, Institut National de
la Recherche Agronomique, Villa Thuret, BP 2078, 06 606 Antibes cedex,
France (M.P., P.R.)
Elicitins are a family of small
proteins secreted by Phytophthora species that have a
high degree of homology and elicit defense reactions in tobacco
(Nicotiana tabacum). They display acidic or basic
characteristics, the acidic elicitins being less efficient in inducing
plant necrosis. In this study we compared the binding properties of
four elicitins (two basic and two acidic) and early-induced signal
transduction events (Ca2+ influx, extracellular medium
alkalinization, and active oxygen species production). The affinity for
tobacco plasma membrane-binding sites and the number of binding sites
were similar for all four elicitins. Furthermore, elicitins compete
with one another for binding sites, suggesting that they interact with
the same receptor. The four elicitins induced Ca2+ influx,
extracellular medium alkalinization, and the production of active
oxygen species in tobacco cell suspensions, but the intensity and
kinetics of these effects were different from one elicitin to another.
As a general observation the concentrations that induce similar levels
of biological activities were lower for basic elicitins (with the
exception of cinnamomin-induced Ca2+ uptake). The
qualitative similarity of early events induced by elicitins indicates a
common transduction scheme, whereas fine signal transduction tuning is
different in each elicitin.
1
This work was supported by the Institut National
de la Recherche Agronomique and by the Conseil Regional de Bourgogne.
S.B. was supported by a grant from the Ministère de
l'Enseignement Supérieur et de la Recherche.
*
Corresponding author; e-mail lebrun{at}epoisses.inra.fr; fax
33-3-80-63-3178.
Plant Physiol. (1998) 118: 1317-1326
Copyright Clearance Center: 0032-0889/98/118//10
© 1998 American Society of Plant Physiologists
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