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Comparison of Binding Properties and Early Biological Effects of Elicitins in Tobacco Cells1

Stéphane Bourque, Michel Ponchet, Marie-Noëlle Binet, Pierre Ricci, Alain Pugin, and Angela Lebrun-Garcia*

Unité Associée Institut National de la Recherche Agronomique-Université de Bourgogne no. 692, Laboratoire de Phytopharmacie et Biochimie des Interactions Cellulaires, BV 1540, 21 034 Dijon cedex, France (S.B., M.-N.B., A.P., A.L.-G.); and Station de Botanique et de Pathologie Végétale, Institut National de la Recherche Agronomique, Villa Thuret, BP 2078, 06 606 Antibes cedex, France (M.P., P.R.)

Elicitins are a family of small proteins secreted by Phytophthora species that have a high degree of homology and elicit defense reactions in tobacco (Nicotiana tabacum). They display acidic or basic characteristics, the acidic elicitins being less efficient in inducing plant necrosis. In this study we compared the binding properties of four elicitins (two basic and two acidic) and early-induced signal transduction events (Ca2+ influx, extracellular medium alkalinization, and active oxygen species production). The affinity for tobacco plasma membrane-binding sites and the number of binding sites were similar for all four elicitins. Furthermore, elicitins compete with one another for binding sites, suggesting that they interact with the same receptor. The four elicitins induced Ca2+ influx, extracellular medium alkalinization, and the production of active oxygen species in tobacco cell suspensions, but the intensity and kinetics of these effects were different from one elicitin to another. As a general observation the concentrations that induce similar levels of biological activities were lower for basic elicitins (with the exception of cinnamomin-induced Ca2+ uptake). The qualitative similarity of early events induced by elicitins indicates a common transduction scheme, whereas fine signal transduction tuning is different in each elicitin.

1   This work was supported by the Institut National de la Recherche Agronomique and by the Conseil Regional de Bourgogne. S.B. was supported by a grant from the Ministère de l'Enseignement Supérieur et de la Recherche.
*   Corresponding author; e-mail lebrun{at}epoisses.inra.fr; fax 33-3-80-63-3178.

Plant Physiol. (1998) 118: 1317-1326
Copyright Clearance Center:   0032-0889/98/118//10
© 1998 American Society of Plant Physiologists




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