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Distribution of Sulfur within Oilseed Rape Leaves in Response to
Sulfur Deficiency during Vegetative Growth1
Mechteld M.A. Blake-Kalff*,
Kevin R. Harrison,
Malcolm J. Hawkesford,
Fangjie J. Zhao, and
Steve P. McGrath
IACR-Rothamsted, Soil Science Department (M.M.A.B.-K., K.R.H.,
F.J.Z., S.P.M.), and Biochemistry and Physiology Department (M.J.H.),
Harpenden, Hertfordshire AL5 2JQ, United Kingdom
The distribution of S to sulfate,
glucosinolates, glutathione, and the insoluble fraction within oilseed
rape (Brassica napus L.) leaves of different ages was
investigated during vegetative growth. The concentrations of
glutathione and glucosinolates increased from the oldest to the
youngest leaves, whereas the opposite was observed for
SO42 . The concentration of insoluble S was
similar among all of the leaves. At sufficient S supply and in the
youngest leaves, 2% of total S was allocated to glutathione, 6% to
glucosinolates, 50% to the insoluble fraction, and the remainder
accumulated as SO42. In the middle and oldest
leaves, 70% to 90% of total S accumulated as
SO42, whereas glutathione and glucosinolates
together accounted for less than 1% of S. When the S supply was
withdrawn (minus S), the concentrations of all S-containing compounds,
particularly SO42, decreased in the youngest
and middle leaves. Neither glucosinolates nor glutathione were major
sources of S during S deficiency. Plants grown on nutrient solution
containing minus S and low N were less deficient than plants
grown on solution containing minus S and high N. The effect of N was
explained by differences in growth rate. The different responses of
leaves of different ages to S deficiency have to be taken into account
for the development of field diagnostic tests to determine whether
plants are S deficient.
1
This research was supported by the Home-Grown
Cereals Authority (grant no. 015/1/96/OS08/1/96). IACR receives
grant-aided support from the Biotechnology and Biological Science
Research Council of the United Kingdom.
*
Corresponding author; e-mail kalff{at}bbsrc.ac.uk; fax
44-1582-760981.
Plant Physiol. (1998) 118: 1337-1344
Copyright Clearance Center: 0032-0889/98/118//08
© 1998 American Society of Plant Physiologists
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