Plant Physiol. Drug Metab Dispos
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Regulation of Apoplastic NH4+ Concentration in Leaves of Oilseed Rape1

Kent Høier Nielsen* and Jan Kofod Schjoerring

Plant Nutrition Laboratory, Department of Agricultural Sciences, Royal Veterinary and Agricultural University, Thorvaldsensvej 40, DK-1871 Frederiksberg C, Copenhagen, Denmark

Regulation of apoplastic NH4+ concentration in leaves of oilseed rape (Brassica napus L.) was studied using a vacuum-infiltration technique that allowed controlled manipulations of the apoplastic solution. In leaves infiltrated with NH4+-free solution, the apoplastic NH4+ concentration returned in less than 1.5 min to the preinfiltration level of 0.8 mM. Infiltrated 15NH4+ was rapidly diluted by 14NH4+/14NH3 effluxed from the cell. The exchange rate of 15N/14N over the apoplast due to combined 14N efflux from the symplast and 15N influx from the apoplastic solution was 29.4 µmol g-1 fresh weight h-1 between 0 and 5 min after infiltration. The net uptake of NH4+ into the leaf cells increased linearly with apoplastic NH4+ concentrations between 2 and 10 mM and could be partially inhibited by the channel inhibitors La3+ and tetraethylammonium and by Na+ and K+. When apoplastic pH increased from 5.0 to 8.0, the steady-state apoplastic NH4+ concentration decreased from 1.0 to 0.3 mM. Increasing temperature increased the rate of NH4+ net uptake and reduced the apoplastic steady-state NH4+ concentration. We conclude that the apoplastic solution in leaves of oilseed rape constitutes a highly dynamic NH4+ pool.

1   This work was supported by grants from the Danish Agricultural and Veterinary Research Council and The Strategic Environmental Research Program II to J.K.S.
*   Corresponding author; e-mail khn{at}kvl.dk; fax 45-35-283-460.

Plant Physiol. (1998) 118: 1361-1368
Copyright Clearance Center:   0032-0889/98/118//08
© 1998 American Society of Plant Physiologists




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