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Biosynthesis of Camalexin from Tryptophan Pathway Intermediates in Cell-Suspension Cultures of Arabidopsis1

Michael Zook*

Department of Botany and Plant Pathology, Michigan State University, East Lansing, Michigan 48824

Camalexin (3-thiazol-2'-yl-indole) is the principal phytoalexin that accumulates in Arabidopsis after infection by fungi or bacteria. Camalexin accumulation was detectable in Arabidopsis cell-suspension cultures 3 to 5 h after inoculation with Cochliobolus carbonum (Race 1), and then increased rapidly from 7 to 24 h after inoculation. Levels of radioactivity incorporated into camalexin during a 1.5-h pulse labeling with [14C]anthranilate also increased with time after fungal inoculation. The levels of radioactive incorporation into camalexin increased rapidly between 7 and 18 h after inoculation, and then decreased along with camalexin accumulation. Relatively low levels of radioactivity from [14C]anthranilate incorporated into camalexin in the noninoculated controls. Autoradiographic analysis of the accumulation of chloroform-extractable metabolites labeled with [14C]anthranilate revealed a transient increase in the incorporation of radioactivity into indole in fungus-inoculated Arabidopsis cell cultures. The time-course measurement of radioactive incorporation into camalexin during a 1.5-h pulse labeling with [14C]indole was similar to that with [14C]anthranilate. These data suggest that indole destined for camalexin synthesis is produced by a separate enzymatic reaction that does not involve tryptophan synthase.

1   This research was supported in part by the Michigan State University Agricultural Experiment Station and by grant no. IBN-9220912 from the National Science Foundation.
*   Dr. Zook passed away in July 1998. Please address all correspondence to: Dr. Ray Hammerschmidt, Department of Botany and Plant Pathology, Michigan State University, East Lansing, MI 48824; e-mail hammerS1{at}pilot.msu.edu; fax 1-517-353-1926.

Plant Physiol. (1998) 118: 1389-1393
Copyright Clearance Center:   0032-0889/98/118//05
© 1998 American Society of Plant Physiologists




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