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The First Step of Gibberellin Biosynthesis in Pumpkin Is
Catalyzed by at Least Two Copalyl Diphosphate Synthases Encoded by
Differentially Regulated Genes
Maria W. Smith*,
Shinjiro Yamaguchi1,
Tahar Ait-Ali2, and
Yuji Kamiya
Plant Hormone Function Team, Frontier Research Program, The
Institute of Physical and Chemical Research (RIKEN), Hirosawa 2-1,
Wako-shi, Saitama 351-0198, Japan
The
first step in gibberellin biosynthesis is catalyzed by copalyl
diphosphate synthase (CPS) and ent-kaurene synthase. We have cloned from pumpkin (Cucurbita maxima L.) two
cDNAs, CmCPS1 and CmCPS2, that each
encode a CPS. Both recombinant fusion CmCPS proteins
were active in vitro. CPS are translocated into plastids and processed
by cleavage of transit peptides. For CmCPS1 and CmCPS2, the putative transit peptides cannot exceed the
first 99 and 107 amino acids, respectively, because longer N-terminal deletions abolished activity. Levels of both CmCPS
transcripts were strictly regulated in an organ-specific and
developmental manner. Both transcripts were almost undetectable in
leaves and were abundant in petioles. CmCPS1 transcript
levels were high in young cotyledons and low in roots. In contrast,
CmCPS2 transcripts were undetectable in cotyledons but
present at significant levels in roots. In hypocotyls, apices, and
petioles, CmCPS1 transcript levels decreased with age
much more rapidly than those of CmCPS2. We speculate that CmCPS1 expression is
correlated with the early stages of organ development, whereas
CmCPS2 expression is correlated with subsequent growth.
In contrast, C. maxima ent-kaurene synthase transcripts
were detected in every organ at almost constant levels. Thus,
ent-kaurene biosynthesis may be regulated through
control of CPS expression.
1
Present address: Developmental, Cell and
Molecular Biology Group, Department of Botany, Box 91000, Duke
University, Durham, NC 27708-1000.
2
Present address: Department of Molecular
Genetics, John Innes Centre, Colney Lane, Norwich NR4 7UJ, UK.
*
Corresponding author; e-mail smimasha{at}postman.riken.go.jp; fax
81-048-462-4691.
Plant Physiol. (1998) 118: 1411-1419
Copyright Clearance Center: 0032-0889/98/118//09
© 1998 American Society of Plant Physiologists
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