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Differential Responses of Abaxial and Adaxial Guard Cells of Broad Bean to Abscisic Acid and Calcium1

Xi-Qing Wang, Wei-Hua Wu*, and Sarah M. Assmann

Department of Plant Physiology and Biochemistry, College of Biological Sciences, China Agricultural University, Beijing 100094, China (W.-H.W.); Department of Basic Sciences, Northwestern Agricultural University, Yang-Ling, Shaanxi Province 712100, China (X.-Q.W.); and Department of Biology, Pennsylvania State University, University Park, Pennsylvania 16802 (S.M.A.)

Regulation by abscisic acid (ABA) and Ca2+ of broad bean (Vicia faba) abaxial and adaxial guard cell movements and inward K+ currents were compared. One millimolar Ca2+ in the bathing medium inhibited abaxial stomatal opening by 60% but only inhibited adaxial stomatal opening by 15%. The addition of 1 µM ABA in the bathing medium resulted in 80% inhibition of abaxial but only 45% inhibition of adaxial stomatal opening. Similarly, ABA and Ca2+ each stimulated greater abaxial stomatal closure than adaxial stomatal closure. Whole-cell patch-clamp results showed that the inward K+ currents of abaxial guard cells were inhibited by 60% (-180 mV) in the presence of 1.5 µM Ca2+ in the cytoplasm, whereas the inward K+ currents of adaxial guard cells were not affected at all by the same treatment. Although 1 µM ABA in the cytoplasm inhibited the inward K+ currents to a similar extent for both abaxial and adaxial guard cells, the former were more sensitive to ABA applied externally. These results suggest that the abaxial stomata are more sensitive to Ca2+ and ABA than adaxial stomata in regard to stomatal opening and closing processes and that the regulation of the inward K+ currents by ABA may not proceed via a Ca2+-signaling pathway in adaxial guard cells. Therefore, there may be different pathways for ABA- and Ca2+-mediated signal transduction in abaxial and adaxial guard cells.

1   This study was supported by a National Outstanding Young Scientist grant (no. 39525003) and a Competitive Research grant (no. 39470359 to W.-H.W.) from the National Science Foundation of China. X.-Q.W. was partially supported by a Ph.D. program grant to Northwestern Agricultural University from the National Educational Commission of China.
*   Corresponding author; e-mail wuwh{at}public3.bta.net.cn; fax 8610-6289-3450.

Plant Physiol. (1998) 118: 1421-1429
Copyright Clearance Center:   0032-0889/98/118//09
© 1998 American Society of Plant Physiologists




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