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An in Vitro System from Maize Seedlings for Tryptophan-Independent Indole-3-Acetic Acid Biosynthesis1

Anders Östin, Neboj<A><AC>s</AC><AC>&cjs1171;</AC></A>a Ilic', and Jerry D. Cohen*

Department of Forest Genetics and Plant Physiology, The Swedish University of Agricultural Sciences, 90183 Umeå, Sweden (A.Ö.); Department of Plant Biology, University of Maryland, College Park, Maryland 20742 (N.I.); and Horticultural Crops Quality Laboratory, Beltsville Agricultural Research Center, Agricultural Research Service, United States Department of Agriculture, Beltsville, Maryland 20705-2350 (J.D.C.)

The enzymatic synthesis of indole-3-acetic acid (IAA) from indole by an in vitro preparation from maize (Zea mays L.) that does not use tryptophan (Trp) as an intermediate is described. Light-grown seedlings of normal maize and the maize mutant orange pericarp were shown to contain the necessary enzymes to convert [14C]indole to IAA. The reaction was not inhibited by unlabeled Trp and neither [14C]Trp nor [14C]serine substituted for [14C]indole in this in vitro system. The reaction had a pH optimum greater than 8.0, required a reducing environment, and had an oxidation potential near that of ascorbate. The results obtained with this in vitro enzyme preparation provide strong, additional evidence for the presence of a Trp-independent IAA biosynthesis pathway in plants.

1   This study was supported by the U.S. Department of Energy (grant no. DE-AI02-94ER20153) and by the Swedish Council for Forestry and Agricultural Research.
*   Corresponding author; e-mail jdcohen{at}wam.umd.edu; fax 1-301-504-5107.

Plant Physiol. (1999) 119: 173-178
Copyright Clearance Center:   0032-0889/99/119//06
© 1999 American Society of Plant Physiologists




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