Interaction of Osmotic Stress, Temperature, and Abscisic Acid in the Regulation of Gene Expression in Arabidopsis

Liming Xiong, Manabu Ishitani, and Jian-Kang Zhu^*

Department of Plant Sciences, University of Arizona, Tucson, Arizona 85721

The impact of simultaneous environmental stresses on plants and how they respond to combined stresses compared with single stresses is largely unclear. By using a transgene (RD29A-LUC) consisting of the firefly luciferase coding sequence (LUC) driven by the stress-responsive RD29A promoter, we investigated the interactive effects of temperature, osmotic stress, and the phytohormone abscisic acid (ABA) in the regulation of gene expression in Arabidopsis seedlings. Results indicated that both positive and negative interactions exist among the studied stress factors in regulating gene expression. At a normal growth temperature (22°C), osmotic stress and ABA act synergistically to induce the transgene expression. Low temperature inhibits the response to osmotic stress or to combined treatment of osmotic stress and ABA, whereas low temperature and ABA treatments are additive in inducing transgene expression. Although high temperature alone does not activate the transgene, it significantly amplifies the effects of ABA and osmotic stress. The effect of multiple stresses in the regulation of RD29A-LUC expression in signal transduction mutants was also studied. The results are discussed in the context of cold and osmotic stress signal transduction pathways.

Plant Physiol. (1999) 119: 205-212
Copyright Clearance Center:   0032-0889/99/119//08
© 1999 American Society of Plant Physiologists

This article has been cited by other articles:

				B.-h. Lee and J.-K. Zhu Phenotypic Analysis of Arabidopsis Mutants: Root Elongation under Salt/Hormone-Induced Stress CSH Protocols, November 1, 2009; 2009(11): pdb.prot4968 - pdb.prot4968. [Abstract] [Full Text]

				I. J. Tetlow, K. G. Beisel, S. Cameron, A. Makhmoudova, F. Liu, N. S. Bresolin, R. Wait, M. K. Morell, and M. J. Emes Analysis of Protein Complexes in Wheat Amyloplasts Reveals Functional Interactions among Starch Biosynthetic Enzymes Plant Physiology, April 1, 2008; 146(4): 1878 - 1891. [Abstract] [Full Text] [PDF]

				X. Zhang, A. M. Myers, and M. G. James Mutations Affecting Starch Synthase III in Arabidopsis Alter Leaf Starch Structure and Increase the Rate of Starch Synthesis Plant Physiology, June 1, 2005; 138(2): 663 - 674. [Abstract] [Full Text] [PDF]

				P. Boominathan, R. Shukla, A. Kumar, D. Manna, D. Negi, P. K. Verma, and D. Chattopadhyay Long Term Transcript Accumulation during the Development of Dehydration Adaptation in Cicer arietinum Plant Physiology, July 1, 2004; 135(3): 1608 - 1620. [Abstract] [Full Text] [PDF]

				M. J. d. Sousa-Majer, N. C. Turner, D. C. Hardie, R. L. Morton, B. Lamont, and T. J. V. Higgins Response to water deficit and high temperature of transgenic peas (Pisum sativum L.) containing a seed-specific {alpha}-amylase inhibitor and the subsequent effects on pea weevil (Bruchus pisorum L.) survival J. Exp. Bot., February 1, 2004; 55(396): 497 - 505. [Abstract] [Full Text] [PDF]

				E. Zocchi, G. Basile, C. Cerrano, G. Bavestrello, M. Giovine, S. Bruzzone, L. Guida, A. Carpaneto, R. Magrassi, and C. Usai ABA- and cADPR-mediated effects on respiration and filtration downstream of the temperature-signaling cascade in sponges J. Cell Sci., February 15, 2003; 116(4): 629 - 636. [Abstract] [Full Text] [PDF]

				L. Rizhsky, H. Liang, and R. Mittler The Combined Effect of Drought Stress and Heat Shock on Gene Expression in Tobacco Plant Physiology, November 1, 2002; 130(3): 1143 - 1151. [Abstract] [Full Text] [PDF]

				A. Welling, T. Moritz, E. T. Palva, and O. Junttila Independent Activation of Cold Acclimation by Low Temperature and Short Photoperiod in Hybrid Aspen Plant Physiology, August 1, 2002; 129(4): 1633 - 1641. [Abstract] [Full Text] [PDF]

				L. Xiong, K. S. Schumaker, and J.-K. Zhu Cell Signaling during Cold, Drought, and Salt Stress PLANT CELL, May 1, 2002; 14(90001): S165 - 183. [Full Text] [PDF]

				N. A. Eckardt Luc Genetic Screen Illuminates Stress-Responsive Gene Regulation PLANT CELL, September 1, 2001; 13(9): 1969 - 1972. [Full Text] [PDF]

				L. Xiong, M. Ishitani, H. Lee, and J.-K. Zhu The Arabidopsis LOS5/ABA3 Locus Encodes a Molybdenum Cofactor Sulfurase and Modulates Cold Stress- and Osmotic Stress-Responsive Gene Expression PLANT CELL, September 1, 2001; 13(9): 2063 - 2083. [Abstract] [Full Text] [PDF]

				J. C. Cushman Osmoregulation in Plants: Implications for Agriculture Integr. Comp. Biol., August 1, 2001; 41(4): 758 - 769. [Abstract] [Full Text] [PDF]

				C. Pinheiro, M. M. Chaves, and C. P. Ricardo Alterations in carbon and nitrogen metabolism induced by water deficit in the stems and leaves of Lupinus albus L. J. Exp. Bot., May 1, 2001; 52(358): 1063 - 1070. [Abstract] [Full Text] [PDF]

				E. Vranova, C. Langebartels, M. Van Montagu, D. Inze, and W. Van Camp Oxidative stress, heat shock and drought differentially affect expression of a tobacco protein phosphatase 2C J. Exp. Bot., October 1, 2000; 51(351): 1763 - 1764. [Abstract] [Full Text] [PDF]

				H. Chiron, A. Drouet, F. Lieutier, H.-D. Payer, D. Ernst, and H. Sandermann Jr. Gene Induction of Stilbene Biosynthesis in Scots Pine in Response to Ozone Treatment, Wounding, and Fungal Infection Plant Physiology, October 1, 2000; 124(2): 865 - 872. [Abstract] [Full Text]

				S. Y. Fujimoto, M. Ohta, A. Usui, H. Shinshi, and M. Ohme-Takagi Arabidopsis Ethylene-Responsive Element Binding Factors Act as Transcriptional Activators or Repressors of GCC Box-Mediated Gene Expression PLANT CELL, March 1, 2000; 12(3): 393 - 404. [Abstract] [Full Text]