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Temporal and Spatial Patterns of Accumulation of the Transcript of Myo-Inositol-1-Phosphate Synthase and Phytin-Containing Particles during Seed Development in Rice1

Kaoru T. Yoshida, Tomikichi Wada, Hiroshi Koyama2, Ritsuko Mizobuchi-Fukuoka3, and Satoshi Naito*

Department of Agricultural and Environmental Biology, Graduate School of Agricultural and Life Sciences, University of Tokyo, Tokyo 113-8657, Japan (K.T.Y., H.K., R.M.-F.); School of Agricultural Science, Nagoya University, Nagoya 464-8601, Japan (T.W.); and Department of Applied Bioscience, Faculty of Agriculture, Hokkaido University, Sapporo 060-8589, Japan (S.N.)

Myo-inositol-1-phosphate (I[1]P) synthase (EC 5.5.1.4) catalyzes the reaction from glucose 6-phosphate to I(1)P, the first step of myo-inositol biosynthesis. Among the metabolites of I(1)P is inositol hexakisphosphate, which forms a mixed salt called phytin or phytate, a storage form of phosphate and cations in seeds. We have isolated a rice (Oryza sativa L.) cDNA clone, pRINO1, that is highly homologous to the I(1)P synthase from yeast and plants. Northern analysis of total RNA showed that the transcript accumulated to high levels in embryos but was undetectable in shoots, roots, and flowers. In situ hybridization of developing seeds showed that the transcript first appeared in the apical region of globular-stage embryos 2 d after anthesis (DAA). Strong signals were detected in the scutellum and aleurone layer after 4 DAA. The level of the transcript in these cells increased until 7 DAA, after which time it gradually decreased. Phytin-containing particles called globoids appeared 4 DAA in the scutellum and aleurone layer, coinciding with the localization of the RINO1 transcript. The temporal and spatial patterns of accumulation of the RINO1 transcript and globoids suggest that I(1)P synthase directs phytin biosynthesis in rice seeds.

1   This work was supported in part by a Grant-in-Aid for Scientific Research from the Ministry of Education, Science and Culture of Japan to K.T.Y.
2   Present address: Kyushu National Agricultural Experimental Station, Chikugo 833-0041, Japan.
3   Present address: Nakano Central Research Institute, Nakano Vinegar Co., Handa 475-8585, Japan.
*   Corresponding author; e-mail naito{at}abs.agr.hokudai.ac.jp; fax 81-11-706-4932.

Plant Physiol. (1999) 119: 65-72
Copyright Clearance Center:   0032-0889/99/119//08
© 1999 American Society of Plant Physiologists




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