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In Vitro Biosynthesis of Phosphorylated Starch in Intact Potato Amyloplasts1

Bente Wischmann*, Tom Hamborg Nielsen, and Birger Lindberg Møller

Plant Biochemistry Laboratory, Department of Plant Biology, The Royal Veterinary and Agricultural University, 40 Thorvaldsensvej, DK-1871 Frederiksberg C, Copenhagen, Denmark

Intact amyloplasts from potato (Solanum tuberosum L.) were used to study starch biosynthesis and phosphorylation. Assessed by the degree of intactness and by the level of cytosolic and vacuolar contamination, the best preparations were selected by searching for amyloplasts containing small starch grains. The isolated, small amyloplasts were 80% intact and were free from cytosolic and vacuolar contamination. Biosynthetic studies of the amyloplasts showed that [1-14C]glucose-6-phosphate (Glc-6-P) was an efficient precursor for starch synthesis in a manner highly dependent on amyloplast integrity. Starch biosynthesis from [1-14C]Glc-1-P in small, intact amyloplasts was 5-fold lower and largely independent of amyloplast intactness. When [33P]Glc-6-P was administered to the amyloplasts, radiophosphorylated starch was produced. Isoamylase treatment of the starch followed by high-performance anion-exchange chromatography with pulsed amperometric detection revealed the separated phosphorylated alpha -glucans. Acid hydrolysis of the phosphorylated alpha -glucans and high-performance anion-exchange chromatography analyses showed that the incorporated phosphate was preferentially positioned at C-6 of the Glc moiety. The incorporation of radiolabel from Glc-1-P into starch in preparations of amyloplasts containing large grains was independent of intactness and most likely catalyzed by starch phosphorylase bound to naked starch grains.

1   This study was supported by The Danish Center for Plant Biotechnology, by the FØTEK Program, and by The Nordic Fund for Technology and Industrial Development.
*   Corresponding author; e-mail bw{at}kvl.dk; fax 45-3528-3333.

Plant Physiol. (1999) 119: 455-462
Copyright Clearance Center:   0032-0889/99/119//08
© 1999 American Society of Plant Physiologists




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