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Arabidopsis ent-Kaurene Oxidase Catalyzes Three Steps of Gibberellin Biosynthesis

Chris A. Helliwell*, Andrew Poole, W. James Peacock, and Elizabeth S. Dennis

Commonwealth Scientific and Industrial Research Organization, Plant Industry, G.P.O. Box 1600, Canberra, ACT 2601, Australia

The Arabidopsis GA3 cDNA was expressed in yeast (Saccharomyces cerevisiae) and the ability of the transformed yeast cells to metabolize ent-kaurene was tested. We show by full-scan gas chromatography-mass spectrometry that the transformed cells produce ent-kaurenoic acid, and demonstrate that the single enzyme GA3 (ent-kaurene oxidase) catalyzes the three steps of gibberellin biosynthesis from ent-kaurene to ent-kaurenoic acid.

*   Corresponding author; e-mail chrish{at}pican.pi.csiro.au; fax 61-2-6246-5000.

Plant Physiol. (1999) 119: 507-510
Copyright Clearance Center:   0032-0889/99/119//04
© 1999 American Society of Plant Physiologists




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