|
The Interaction between Cold and Light Controls the Expression of the
Cold-Regulated Barley Gene cor14b and the Accumulation of
the Corresponding Protein1
Cristina Crosatti*,
Patrizia Polverino de Laureto,
Roberto Bassi, and
Luigi Cattivelli
Istituto Sperimentale per la Cerealicoltura, Via S. Protaso 302, I-29017, Fiorenzuola d'Arda (PC), Italy (C.C., L.C.); Centro de
Ricerca Interdipartimentale Biotecnologie Innovative, Università
di Padova, via Trieste 45, 35121, Padova, Italy (P.P.d.L.); and Università di Verona, Facoltà di Scienze Matematiche,
Fisiche é Naturali, Biotecnologie Vegetali, Strada Le
Grazie, 37134, Verona, Italy (R.B.)
We report the expression of the
barley (Hordeum vulgare L.) COR
(cold-regulated) gene
cor14b (formerly pt59) and the
accumulation of its chloroplast-localized protein product. A polyclonal
antibody raised against the cor14b-encoded protein
detected two chloroplast COR proteins: COR14a and COR14b. N-terminal
sequencing of COR14a and expression of cor14b in
Arabidopsis plants showed that COR14a is not encoded by the
cor14b sequence, but it shared homology with the wheat
(Triticum aestivum L.) WCS19 COR protein. The expression of cor14b was strongly impaired in the barley albino
mutant an, suggesting the involvement of a
plastidial factor in the control of gene expression. Low-level
accumulation of COR14b was induced by cold treatment in etiolated
plants, although cor14b expression and protein
accumulation were enhanced after a short light pulse. Light quality was
a determining factor in regulating gene expression: red or blue but not
far-red or green light pulses were able to promote COR14b accumulation
in etiolated plants, suggesting that phytochrome and blue light
photoreceptors may be involved in the control of cor14b
gene expression. Maximum accumulation of COR14b was reached only when
plants were grown and/or hardened under the standard photoperiod. The
effect of light on the COR14b stability was demonstrated by using
transgenic Arabidopsis. These plants constitutively expressed
cor14b mRNAs regardless of temperature and light
conditions; nevertheless, green plants accumulated about twice as much
COR14b protein as etiolated plants.
1
This work was funded by Ministero per le
Politiche Agricole, "Progetto Biotecnologie Vegetali" and by
Consiglio Nazionale delle Richerche "Progetto Biotecnologie."
*
Corresponding author; e-mail iscfior{at}agonet.it; fax
39-0523-983750.
Plant Physiol. (1999) 119: 671-680
Copyright Clearance Center: 0032-0889/99/119//10
© 1999 American Society of Plant Physiologists
This article has been cited by other articles:
|
|
|
|
 
M. Rapacz, B. Wolanin, K. Hura, and M. Tyrka
The Effects of Cold Acclimation on Photosynthetic Apparatus and the Expression of COR14b in Four Genotypes of Barley (Hordeum vulgare) Contrasting in their Tolerance to Freezing and High-light Treatment in Cold Conditions
Ann. Bot.,
April 1, 2008;
101(5):
689 - 699.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S. H. Hung, C. C. Wang, S. V. Ivanov, V. Alexieva, and C. W. Yu
Repetition of Hydrogen Peroxide Treatment Induces a Chilling Tolerance Comparable to Cold Acclimation in Mung Bean
J. Amer. Soc. Hort. Sci.,
November 1, 2007;
132(6):
770 - 776.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
K. Nakayama, K. Okawa, T. Kakizaki, T. Honma, H. Itoh, and T. Inaba
Arabidopsis Cor15am Is a Chloroplast Stromal Protein That Has Cryoprotective Activity and Forms Oligomers
Plant Physiology,
May 1, 2007;
144(1):
513 - 523.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J. Cockram, H. Jones, F. J. Leigh, D. O'Sullivan, W. Powell, D. A. Laurie, and A. J. Greenland
Control of flowering time in temperate cereals: genes, domestication, and sustainable productivity
J. Exp. Bot.,
April 9, 2007;
(2007)
erm042v1.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J. T. Svensson, C. Crosatti, C. Campoli, R. Bassi, A. M. Stanca, T. J. Close, and L. Cattivelli
Transcriptome Analysis of Cold Acclimation in Barley Albina and Xantha Mutants
Plant Physiology,
May 1, 2006;
141(1):
257 - 270.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
L. V. Savitch, G. Allard, M. Seki, L. S. Robert, N. A. Tinker, N. P. A. Huner, K. Shinozaki, and J. Singh
The Effect of Overexpression of Two Brassica CBF/DREB1-like Transcription Factors on Photosynthetic Capacity and Freezing Tolerance in Brassica napus
Plant Cell Physiol.,
September 1, 2005;
46(9):
1525 - 1539.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S. Takumi, A. Koike, M. Nakata, S. Kume, R. Ohno, and C. Nakamura
Cold-specific and light-stimulated expression of a wheat (Triticum aestivum L.) Cor gene Wcor15 encoding a chloroplast-targeted protein
J. Exp. Bot.,
October 1, 2003;
54(391):
2265 - 2274.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
C. Dal Bosco, M. Busconi, C. Govoni, P. Baldi, A. M. Stanca, C. Crosatti, R. Bassi, and L. Cattivelli
cor Gene Expression in Barley Mutants Affected in Chloroplast Development and Photosynthetic Electron Transport
Plant Physiology,
February 1, 2003;
131(2):
793 - 802.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
C. Finnie, S. Melchior, P. Roepstorff, and B. Svensson
Proteome Analysis of Grain Filling and Seed Maturation in Barley
Plant Physiology,
July 1, 2002;
129(3):
1308 - 1319.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
C. NDong, J. Danyluk, K. E. Wilson, T. Pocock, N. P.A. Huner, and F. Sarhan
Cold-Regulated Cereal Chloroplast Late Embryogenesis Abundant-Like Proteins. Molecular Characterization and Functional Analyses
Plant Physiology,
July 1, 2002;
129(3):
1368 - 1381.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
D. B. Fowler, G. Breton, A. E. Limin, S. Mahfoozi, and F. Sarhan
Photoperiod and Temperature Interactions Regulate Low-Temperature-Induced Gene Expression in Barley
Plant Physiology,
December 1, 2001;
127(4):
1676 - 1681.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S. D. Lahiri and L. A. Allison
Complementary Expression of Two Plastid-Localized sigma -Like Factors in Maize
Plant Physiology,
July 1, 2000;
123(3):
883 - 894.
[Abstract]
[Full Text]
|
|
|
|
|
