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Differential Expression of Three Members of the
1-Aminocyclopropane-1-Carboxylate Synthase Gene
Family in
Carnation1
Michelle L. Jones2 and
William R. Woodson*
Department of Horticulture and Landscape Architecture, Purdue
University, West Lafayette, Indiana 47907-1165
We
investigated the expression patterns of three
1-aminocyclopropane-1-carboxylate (ACC) synthase genes in carnation
(Dianthus caryophyllus cv White Sim) under conditions
previously shown to induce ethylene biosynthesis. These included
treatment of flowers with 2,4-dichlorophenoxyacetic acid, ethylene,
LiCl, cycloheximide, and natural and pollination-induced flower
senescence. Accumulation of ACC synthase transcripts in leaves
following mechanical wounding and treatment with
2,4-dichlorophenoxyacetic acid or LiCl was also determined by RNA
gel-blot analysis. As in other species, the carnation ACC synthase
genes were found to be differentially regulated in a tissue-specific
manner. DCACS2 and DCACS3 were preferentially expressed in styles,
whereas DCACS1 mRNA was most abundant in petals. Cycloheximide did not
induce increased accumulation of ACC synthase transcripts in carnation
flowers, whereas the expression of ACC synthase was up-regulated by
auxin, ethylene, LiCl, pollination, and senescence in a
floral-organ-specific manner. Expression of the three ACC synthases
identified in carnation did not correspond to elevated ethylene
biosynthesis from wounded or auxin-treated leaves, and there are likely
additional members of the carnation ACC synthase gene family
responsible for ACC synthase expression in vegetative tissues.
1
This research was supported by grants from the
U.S. Department of Agriculture/National Research Initiative Competitive
Grants Program (no. 92-37304-7867) and the American Floral Endowment. This is publication no. 15,897 of the Purdue University Office of
Agricultural Research Programs.
2
Present address: Department of Horticulture and
Landscape Architecture, Colorado State University, Fort Collins, CO
80523-1173.
*
Corresponding author; e-mail woodson{at}purdue.edu; fax
1-765-494-0808.
Plant Physiol. (1999) 119: 755-764
Copyright Clearance Center: 0032-0889/99/119//10
© 1999 American Society of Plant Physiologists
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