Plant Physiol. Illumina
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via CrossRef
Right arrow Citing Articles via Web of Science (92)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Pego, J. V.
Right arrow Articles by Smeekens, S. C.M.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Pego, J. V.
Right arrow Articles by Smeekens, S. C.M.
Agricola
Right arrow Articles by Pego, J. V.
Right arrow Articles by Smeekens, S. C.M.

Mannose Inhibits Arabidopsis Germination via a Hexokinase-Mediated Step1

Jónatas V. Pego*, Peter J. Weisbeek, and Sjef C.M. Smeekens

Department of Botanical Ecology and Evolutionary Biology (J.V.P., S.C.M.S.), and Department of Molecular Cell Biology (J.V.P., P.J.W., S.C.M.S.), University of Utrecht, Padualaan 8, 3584 CH Utrecht, The Netherlands

Low concentrations of the glucose (Glc) analog mannose (Man) inhibit germination of Arabidopsis seeds. Man is phosphorylated by hexokinase (HXK), but the absence of germination was not due to ATP or phosphate depletion. The addition of metabolizable sugars reversed the Man-mediated inhibition of germination. Carbohydrate-mediated regulation of gene expression involving a HXK-mediated pathway is known to be activated by Glc, Man, and other monosaccharides. Therefore, we investigated whether Man blocks germination through this system. By testing other Glc analogs, we found that 2-deoxyglucose, which, like Man, is phosphorylated by HXK, also blocked germination; no inhibition was observed with 6-deoxyglucose or 3-O-methylglucose, which are not substrates for HXK. Since these latter two sugars are taken up at a rate similar to that of Man, uptake is unlikely to be involved in the inhibition of germination. Furthermore, we show that mannoheptulose, a specific HXK inhibitor, restores germination of seeds grown in the presence of Man. We conclude that HXK is involved in the Man-mediated repression of germination of Arabidopsis seeds, possibly via energy depletion.

1   This work was financially supported by the Fundação para a Ciência e Tecnologia, Lisbon, Portugal (grant no. PRAXIS XXI/BD/3103/94 to J.V.P.).
*   Corresponding author; e-mail j.pego{at}bio.uu.nl; fax 31-30-2513655.

Plant Physiol. (1999) 119: 1017-1024
Copyright Clearance Center:   0032-0889/99/119//08
© 1999 American Society of Plant Physiologists




This article has been cited by other articles:


Home page
 J Exp BotHome page
K. Yuan and J. Wysocka-Diller
Phytohormone signalling pathways interact with sugars during seed germination and seedling development
J. Exp. Bot., September 1, 2006; 57(12): 3359 - 3367.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
A. Cernac, C. Andre, S. Hoffmann-Benning, and C. Benning
WRI1 Is Required for Seed Germination and Seedling Establishment
Plant Physiology, June 1, 2006; 141(2): 745 - 757.
[Abstract] [Full Text] [PDF]

Home page
 Plant Cell PhysiolHome page
M. Yoine, T. Nishii, and K. Nakamura
Arabidopsis UPF1 RNA Helicase for Nonsense-mediated mRNA Decay is Involved in Seed Size Control and is Essential for Growth
Plant Cell Physiol., May 1, 2006; 47(5): 572 - 580.
[Abstract] [Full Text] [PDF]

Home page
 J Exp BotHome page
I. Couee, C. Sulmon, G. Gouesbet, and A. El Amrani
Involvement of soluble sugars in reactive oxygen species balance and responses to oxidative stress in plants
J. Exp. Bot., February 1, 2006; 57(3): 449 - 459.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
M. A. Villalobos, D. Bartels, and G. Iturriaga
Stress Tolerance and Glucose Insensitive Phenotypes in Arabidopsis Overexpressing the CpMYB10 Transcription Factor Gene
Plant Physiology, May 1, 2004; 135(1): 309 - 324.
[Abstract] [Full Text] [PDF]

Home page
 J Exp BotHome page
K. Maurel, S. Sakr, F. Gerbe, A. Guilliot, M. Bonhomme, R. Rageau, and G. Petel
Sorbitol uptake is regulated by glucose through the hexokinase pathway in vegetative peach-tree buds
J. Exp. Bot., April 1, 2004; 55(398): 879 - 888.
[Abstract] [Full Text] [PDF]

Home page
 J Exp BotHome page
S. I. Gibson
Sugar and phytohormone response pathways: navigating a signalling network
J. Exp. Bot., January 2, 2004; 55(395): 253 - 264.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
M. Baier, G. Hemmann, R. Holman, F. Corke, R. Card, C. Smith, F. Rook, and M. W. Bevan
Characterization of Mutants in Arabidopsis Showing Increased Sugar-Specific Gene Expression, Growth, and Developmental Responses
Plant Physiology, January 1, 2004; 134(1): 81 - 91.
[Abstract] [Full Text] [PDF]

Home page
 Plant Cell PhysiolHome page
E. Urbanczyk-Wochniak, A. Leisse, U. Roessner-Tunali, A. Lytovchenko, J. Reismeier, L. Willmitzer, and A. R. Fernie
Expression of a Bacterial Xylose Isomerase in Potato Tubers Results in an Altered Hexose Composition and a Consequent Induction of Metabolism
Plant Cell Physiol., December 15, 2003; 44(12): 1359 - 1367.
[Abstract] [Full Text] [PDF]

Home page
 GeneticsHome page
E. Nambara, M. Suzuki, S. Abrams, D. R. McCarty, Y. Kamiya, and P. McCourt
A Screen for Genes That Function in Abscisic Acid Signaling in Arabidopsis thaliana
Genetics, July 1, 2002; 161(3): 1247 - 1255.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
C. Lu and M. J. Hills
Arabidopsis Mutants Deficient in Diacylglycerol Acyltransferase Display Increased Sensitivity to Abscisic Acid, Sugars, and Osmotic Stress during Germination and Seedling Development
Plant Physiology, July 1, 2002; 129(3): 1352 - 1358.
[Abstract] [Full Text] [PDF]

Home page
 Plant CellHome page
F. Rolland, B. Moore, and J. Sheen
Sugar Sensing and Signaling in Plants
PLANT CELL, May 1, 2002; 14(90001): S185 - 205.
[Full Text] [PDF]

Home page
 J Exp BotHome page
M. J. Paul and C. H. Foyer
Sink regulation of photosynthesis
J. Exp. Bot., July 1, 2001; 52(360): 1383 - 1400.
[Abstract] [Full Text] [PDF]

Home page
 J Exp BotHome page
W. S. da-Silva, G. L. Rezende, and A. Galina
Subcellular distribution and kinetic properties of cytosolic and non-cytosolic hexokinases in maize seedling roots: implications for hexose phosphorylation
J. Exp. Bot., June 1, 2001; 52(359): 1191 - 1201.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
A. R. Fernie, U. Roessner, and P. Geigenberger
The Sucrose Analog Palatinose Leads to a Stimulation of Sucrose Degradation and Starch Synthesis When Supplied to Discs of Growing Potato Tubers
Plant Physiology, April 1, 2001; 125(4): 1967 - 1977.
[Abstract] [Full Text]

Home page
 Plant Physiol.Home page
R. Brouquisse, A. Evrard, D. Rolin, P. Raymond, and C. Roby
Regulation of Protein Degradation and Protease Expression by Mannose in Maize Root Tips. Pi Sequestration by Mannose May Hinder the Study of Its Signaling Properties
Plant Physiology, March 1, 2001; 125(3): 1485 - 1498.
[Abstract] [Full Text]

Home page
 Plant Physiol.Home page
S.-L. Ho, Y.-C. Chao, W.-F. Tong, and S.-M. Yu
Sugar Coordinately and Differentially Regulates Growth- and Stress-Related Gene Expression via a Complex Signal Transduction Network and Multiple Control Mechanisms
Plant Physiology, February 1, 2001; 125(2): 877 - 890.
[Abstract] [Full Text]

Home page
 J Exp BotHome page
J. M. Quintero, R. Molina, J. M. Fournier, M. Benlloch, and J. Ramos
Glucose-induced activation of rubidium transport and water flux in sunflower root systems
J. Exp. Bot., January 1, 2001; 52(354): 99 - 104.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
S. I. Gibson
Plant Sugar-Response Pathways. Part of a Complex Regulatory Web
Plant Physiology, December 1, 2000; 124(4): 1532 - 1539.
[Full Text]

Home page
 Genes Dev.Home page
F. Arenas-Huertero, A. Arroyo, L. Zhou, J. Sheen, and P. León
Analysis of Arabidopsis glucose insensitive mutants, gin5 and gin6, reveals a central role of the plant hormone ABA in the regulation of plant vegetative development by sugar
Genes & Dev., August 15, 2000; 14(16): 2085 - 2096.
[Abstract] [Full Text]

Home page
 Plant Physiol.Home page
R. R. Finkelstein and T. J. Lynch
Abscisic Acid Inhibition of Radicle Emergence But Not Seedling Growth Is Suppressed by Sugars
Plant Physiology, April 1, 2000; 122(4): 1179 - 1186.
[Abstract] [Full Text]

Home page
 J Exp BotHome page
J. V. Pego, A. J. Kortstee, C. Huijser, and S. C.M. Smeekens
Photosynthesis, sugars and the regulation of gene expression
J. Exp. Bot., February 1, 2000; 51(90001): 407 - 416.
[Abstract] [Full Text]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
ASPB Publications PLANT PHYSIOLOGY® THE PLANT CELL
Copyright © 1999 by the American Society of Plant Biologists