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Nicotianamine Chelates Both FeIII and
FeII. Implications for Metal Transport in
Plants1
Nicolaus von Wirén2,
Sukhbinder Klair,
Suhkibar Bansal,
Jean-Francois Briat,
Hicham Khodr,
Takayuki Shioiri,
Roger A. Leigh*, and
Robert C. Hider
Department of Pharmacy, King's College London, Manresa Road,
London SW3 6LX, United Kingdom (N.v.W., S.K., S.B., H.K., R.C.H.); Laboratoire de Biochimie et Physiologie Moléculaire des Plantes,
Institut National de la Recherche Agronomique, Place Viala, F-34060
Montpellier, France (J.-F.B.); Faculty of Pharmaceutical Sciences,
Nagoya City University, Tanabe-dori, Mizuho-ku, Nagoya 467, Japan
(T.S.); and Biochemistry and Physiology Department, IACR-Rothamsted,
Harpenden, Hertfordshire AL5 2JQ, United Kingdom (R.A.L.)
Nicotianamine
(NA) occurs in all plants and chelates metal cations, including
FeII, but reportedly not FeIII. However, a
comparison of the FeII and ZnII affinity
constants of NA and various FeIII-chelating
aminocarboxylates suggested that NA should chelate FeIII.
High-voltage electrophoresis of the FeNA complex formed in the presence
of FeIII showed that the complex had a net charge of 0, consistent with the hexadentate chelation of FeIII.
Measurement of the affinity constant for FeIII yielded a
value of 1020.6, which is greater than that for the
association of NA with FeII (1012.8). However,
capillary electrophoresis showed that in the presence of
FeII and FeIII, NA preferentially chelates
FeII, indicating that the FeIINA complex is
kinetically stable under aerobic conditions. Furthermore, Fe complexes
of NA are relatively poor Fenton reagents, as measured by their ability
to mediate H2O2-dependent oxidation of
deoxyribose. This suggests that NA will have an important role in
scavenging Fe and protecting the cell from oxidative damage. The pH
dependence of metal ion chelation by NA and a typical phytosiderophore,
2 -deoxymugineic acid, indicated that although both have the ability to
chelate Fe, when both are present, 2-deoxymugineic acid dominates the chelation process at acidic pH values, whereas NA dominates at alkaline
pH values. The consequences for the role of NA in the long-distance
transport of metals in the xylem and phloem are discussed.
1
The work was supported by a grant from the
Biotechnology and Biological Sciences Research Council (BBSRC) of the
United Kingdom and by a short-term fellowship to N.v.W. from the joint
BBSRC/Institut National de la Recherche Agronomique collaboration
scheme. IACR is grant-aided by the BBSRC.
2
Present address: Institut für Allgemeine
Botanik, Universität Tübingen, Morgenstelle 1, D-72076
Tübingen, Germany.
*
Corresponding author; e-mail RL225{at}cam.ac.uk; fax 44-1223-333953.
Copies of the computer program mentioned in the paper are available
from R.C.H. or H.K.
Plant Physiol. (1999) 119: 1107-1114
Copyright Clearance Center: 0032-0889/99/119//08
© 1999 American Society of Plant Physiologists
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