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Glycerol Is a Suberin Monomer. New Experimental Evidence for an
Old Hypothesis1
Laurence Moire,
Alain Schmutz,
Antony Buchala,
Bin Yan,
Ruth E. Stark, and
Ulrich Ryser*
Institut für Botanische Biologie, Universität Freiburg,
A. Gockelstrasse 3, CH-1700 Freiburg, Switzerland (L.M., A.S.,
A.B., U.R.); and Department of Chemistry, City University of New York
College of Staten Island, 2800 Victory Boulevard, Staten Island,
New York 10314-6600 (B.Y., R.E.S.)
The monomer composition of the
esterified part of suberin can be determined using gas
chromatography-mass spectroscopy technology and is accordingly believed
to be well known. However, evidence was presented recently indicating
that the suberin of green cotton (Gossypium hirsutum cv
Green Lint) fibers contains substantial amounts of esterified glycerol.
This observation is confirmed in the present report by a sodium dodecyl
sulfate extraction of membrane lipids and by a developmental study,
demonstrating the correlated accumulation of glycerol and established
suberin monomers. Corresponding amounts of glycerol also occur in the
suberin of the periderm of cotton stems and potato (Solanum
tuberosum) tubers. A periderm preparation of wound-healing
potato tuber storage parenchyma was further purified by different
treatments. As the purification proceeded, the concentration of
glycerol increased at about the same rate as that of
 , -alkanedioic acids, the most diagnostic suberin monomers.
Therefore, it is proposed that glycerol is a monomer of suberins in
general and can cross-link aliphatic and aromatic suberin domains,
corresponding to the electron-translucent and electron-opaque suberin
lamellae, respectively. This proposal is consistent with the reported
dimensions of the electron-translucent suberin lamellae.
1
This work was supported by the Swiss National
Science Foundation (grant nos. 31-39648.93 and 31-49305.96 to U.R.) and
by the U.S. National Science Foundation (grant nos. MCB-9406354 and
MCB-9728503 to R.E.S.).
*
Corresponding author; e-mail ulrich.ryser{at}unifr.ch; fax
41-26-300-97-40.
Plant Physiol. (1999) 119: 1137-1146
Copyright Clearance Center: 0032-0889/99/119//10
© 1999 American Society of Plant Physiologists
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