Plant Physiol. Illumina
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via CrossRef
Right arrow Citing Articles via Web of Science (21)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Trepp, G. B.
Right arrow Articles by Vance, C. P.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Trepp, G. B.
Right arrow Articles by Vance, C. P.
Agricola
Right arrow Articles by Trepp, G. B.
Right arrow Articles by Vance, C. P.

NADH-Glutamate Synthase in Alfalfa Root Nodules. Immunocytochemical Localization1

Gian B. Trepp, David W. Plank, J. Stephen Gantt, and Carroll P. Vance*

Institut für Pflanzenwissenschaften Eidgenössische Technische Hochschule-Zürich, 8092 Zurich, Switzerland (G.B.T.); Department of Agronomy and Plant Genetics (G.B.T., D.W.P., C.P.V) and Department of Plant Biology (J.S.G.), University of Minnesota, St. Paul, Minnesota 55108; and University of Minnesota, St. Paul, Minnesota 55108United States Department of Agriculture, Agricultural Research Service, Plant Science Research Unit, University of Minnesota, St. Paul, Minnesota 55108 (C.P.V.)

In root nodules of alfalfa (Medicago sativa L.), N2 is reduced to NH4+ in the bacteroid by the nitrogenase enzyme and then released into the plant cytosol. The NH4+ is then assimilated by the combined action of glutamine synthetase (EC 6.3.1.2) and NADH-dependent Glu synthase (NADH-GOGAT; EC 1.4.1.14) into glutamine and Glu. The alfalfa nodule NADH-GOGAT protein has a 101-amino acid presequence, but the subcellular location of the protein is unknown. Using immunocytochemical localization, we determined first that the NADH-GOGAT protein is found throughout the infected cell region of both 19- and 33-d-old nodules. Second, in alfalfa root nodules NADH-GOGAT is localized predominantly to the amyloplast of infected cells. This finding, together with earlier localization and fractionation studies, indicates that in alfalfa the infected cells are the main location for the initial assimilation of fixed N2.

1   This work was supported in part by National Science Foundation grant no. IBN-9206890 and ETH-Zurich fellowship no. 0-28-001-91. This paper is a joint contribution from the Plant Science Research Unit, U.S. Department of Agriculture, Agricultural Research Service, and the Minnesota Agricultural Experiment Station (paper no. 98-1-13-0101, Scientific Journal Series).
*   Corresponding author; e-mail vance004{at}maroon.tc.umn.edu; fax 1-651-625-5058.

Plant Physiol. (1999) 119: 829-838
Copyright Clearance Center:   0032-0889/99/119//10
© 1999 American Society of Plant Physiologists




This article has been cited by other articles:


Home page
 Plant Physiol.Home page
J. White, J. Prell, E. K. James, and P. Poole
Nutrient Sharing between Symbionts
Plant Physiology, June 1, 2007; 144(2): 604 - 614.
[Full Text] [PDF]

Home page
 J Exp BotHome page
M. F. Suarez, C. Avila, F. Gallardo, F. R. Canton, A. Garcia-Gutierrez, M. G. Claros, and F. M. Canovas
Molecular and enzymatic analysis of ammonium assimilation in woody plants
J. Exp. Bot., April 15, 2002; 53(370): 891 - 904.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Bot.Home page
J. Imsande, M. Berkemeyer, R. Scheibe, U. Schumann, C. Gietl, and R. G. Palmer
A soybean plastid-targeted NADH-malate dehydrogenase: cloning and expression analyses
Am. J. Botany, December 1, 2001; 88(12): 2136 - 2142.
[Abstract] [Full Text] [PDF]

Home page
 J Exp BotHome page
S. Chichkova, J. Arellano, C. P. Vance, and G. Hernandez
Transgenic tobacco plants that overexpress alfalfa NADH-glutamate synthase have higher carbon and nitrogen content
J. Exp. Bot., November 1, 2001; 52(364): 2079 - 2087.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
N. Hirose and T. Yamaya
Okadaic Acid Mimics Nitrogen-Stimulated Transcription of the NADH-Glutamate Synthase Gene in Rice Cell Cultures
Plant Physiology, November 1, 1999; 121(3): 805 - 812.
[Abstract] [Full Text]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
ASPB Publications PLANT PHYSIOLOGY® THE PLANT CELL
Copyright © 1999 by the American Society of Plant Biologists