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Osmotic Water Permeability of Isolated Protoplasts. Modifications during Development1

Tiana Ramahaleo, Raphaël Morillon, Joël Alexandre, and Jean-Paul Lassalles*

Unité Propre de Recherches de l'Enseignement Supérieur Associée Centre National de la Recherche Scientifique 6037, Université de Rouen, Faculté des Sciences, 76821 Mont-Saint-Aignan cedex, France

A transference chamber was developed to measure the osmotic water permeability coefficient (Pos) in protoplasts 40 to 120 µm in diameter. The protoplast was held by a micropipette and submitted to a steep osmotic gradient created in the transference chamber. Pos was derived from the changes in protoplast dimensions, as measured using a light microscope. Permeabilities were in the range 1 to 1000 µm s-1 for the various types of protoplasts tested. The precision for Pos was <= 40%, and within this limit, no asymmetry in the water fluxes was observed. Measurements on protoplasts isolated from 2- to 5-d-old roots revealed a dramatic increase in Pos during root development. A shift in Pos from 10 to 500 µm s-1 occurred within less than 48 h. This phenomenon was found in maize (Zea mays), wheat (Triticum aestivum), and rape (Brassica napus) roots. These results show that early developmental processes modify water-transport properties of the plasma membrane, and that the transference chamber is adapted to the study of water-transport mechanisms in native membranes.

1   R.M. was supported by a grant from the Biopôle Végétal. T.R. was supported by funds from the Ministère de la Recherche et de l'Enseignement Supérieur (France).
*   Corresponding author; e-mail jp.lassalles{at}univ-rouen.fr; fax 33-2-35-70-55-20.

Plant Physiol. (1999) 119: 885-896
Copyright Clearance Center:   0032-0889/99/119//12
© 1999 American Society of Plant Physiologists




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