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Chloroplast-Avoidance Response Induced by High-Fluence Blue Light
in Prothallial Cells of the Fern Adiantum
capillus-veneris as Analyzed by Microbeam
Irradiation1
Takatoshi Kagawa2, * and
Masamitsu Wada
Department of Biology, Faculty of Science, Tokyo Metropolitan
University, Minami Osawa 1-1, Hachioji, Tokyo 192-0397, Japan
Chloroplast
movement was induced by partial cell illumination using a high-fluence
blue microbeam in light-grown and dark-adapted prothallial cells of the
fern Adiantum capillus-veneris. Chloroplasts inside the
illuminated area moved out (high-fluence response [HFR]), whereas
those outside moved toward the irradiated area (low-fluence response
[LFR]), although they stopped moving when they reached the border.
These results indicate that both HFR and LFR signals are generated by
high-fluence blue light of the same area, and that an LFR signal can be
transferred long-distance from the beam spot, although an HFR signal
cannot. The lifetime of the HFR signal was calculated from the traces
of chloroplast movement induced by a brief pulse from a high-fluence
blue microbeam to be about 6 min. This is very short compared with that
of the LFR (30-40 min; T. Kagawa, M. Wada [1994] J Plant Res 107:
389-398). These data indicate that the signal transduction pathways of
the HFR and the LFR must be distinct.
1
This work was partially supported by a
Grant-in-Aid for Scientific Research (no. 09440270 to M.W.) from the
Ministry of Education, Science, Sports and Culture of Japan.
2
Present address: Division of Biological
Regulation, National Institute for Basic Biology, Okazaki 444-8585,
Japan.
*
Corresponding author; e-mail kagawa{at}nibb.ac.jp; fax
81-564-55-7611.
Plant Physiol. (1999) 119: 917-924
Copyright Clearance Center: 0032-0889/99/119//08
© 1999 American Society of Plant Physiologists
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