Plant Physiol. Journal of Pharmacology and Experimental Therapeutics
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via CrossRef
Right arrow Citing Articles via Web of Science (29)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Shin, H.
Right arrow Articles by Brown, R. M.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Shin, H.
Right arrow Articles by Brown, R. M., Jr.
Agricola
Right arrow Articles by Shin, H.
Right arrow Articles by Brown, R. M.

GTPase Activity and Biochemical Characterization of a Recombinant Cotton Fiber Annexin1

Heungsop Shin and R. Malcolm Brown Jr.*

Department of Botany, University of Texas, Austin, Texas, 78713-7640

A cDNA encoding annexin was isolated from a cotton (Gossypium hirsutum) fiber cDNA library. The cDNA was expressed in Escherichia coli, and the resultant recombinant protein was purified. We then investigated some biochemical properties of the recombinant annexin based on the current understanding of plant annexins. An "add-back experiment" was performed to study the effect of the recombinant annexin on beta -glucan synthase activity, but no effect was found. However, it was found that the recombinant annexin could display ATPase/GTPase activities. The recombinant annexin showed much higher GTPase than ATPase activity. Mg2+ was essential for these activities, whereas a high concentration of Ca2+ was inhibitory. A photolabeling assay showed that this annexin could bind GTP more specifically than ATP. The GTP-binding site on the annexin was mapped into the carboxyl-terminal fourth repeat of annexin from the photolabeling experiment using domain-deletion mutants of this annexin. Northern-blot analysis showed that the annexin gene was highly expressed in the elongation stages of cotton fiber differentiation, suggesting a role of this annexin in cell elongation.

1   This work was supported in part by grant no. DE-FG03-94ER20145 from the Division of Energy Biosciences, Department of Energy, and funds from the Johnson & Johnson Centennial Chair to R.M.B.
*   Corresponding author; e-mail rmbrown{at}mail.utexas.edu; fax 1-512-471-3573.

Plant Physiol. (1999) 119: 925-934
Copyright Clearance Center:   0032-0889/99/119//10
© 1999 American Society of Plant Physiologists




This article has been cited by other articles:


Home page
 J. Biol. Chem.Home page
N.-J. Hu, A. M. Yusof, A. Winter, A. Osman, A. K. Reeve, and A. Hofmann
The Crystal Structure of Calcium-bound Annexin Gh1 from Gossypium hirsutum and Its Implications for Membrane Binding Mechanisms of Plant Annexins
J. Biol. Chem., June 27, 2008; 283(26): 18314 - 18322.
[Abstract] [Full Text] [PDF]

Home page
 J Exp BotHome page
J. C. Mortimer, A. Laohavisit, N. Macpherson, A. Webb, C. Brownlee, N. H. Battey, and J. M. Davies
Annexins: multifunctional components of growth and adaptation
J. Exp. Bot., February 10, 2008; (2008) erm344v1.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
X.-B. Li, L. Cai, N.-H. Cheng, and J.-W. Liu
Molecular Characterization of the Cotton GhTUB1 Gene That Is Preferentially Expressed in Fiber
Plant Physiology, October 1, 2002; 130(2): 666 - 674.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
G. B. Clark, A. Sessions, D. J. Eastburn, and S. J. Roux
Differential Expression of Members of the Annexin Multigene Family in Arabidopsis
Plant Physiology, July 1, 2001; 126(3): 1072 - 1084.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
K. Yuasa and M. Maeshima
Purification, Properties, and Molecular Cloning of a Novel Ca2+-Binding Protein in Radish Vacuoles
Plant Physiology, November 1, 2000; 124(3): 1069 - 1078.
[Abstract] [Full Text]

Home page
 Plant CellHome page
S. Kimura, W. Laosinchai, T. Itoh, X. Cui, C. R. Linder, and R. M. Brown , Jr.
Immunogold Labeling of Rosette Terminal Cellulose-Synthesizing Complexes in the Vascular Plant Vigna angularis
PLANT CELL, November 1, 1999; 11(11): 2075 - 2086.
[Abstract] [Full Text]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
ASPB Publications PLANT PHYSIOLOGY® THE PLANT CELL
Copyright © 1999 by the American Society of Plant Biologists