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Chloroplast-Targeted ERD1 Protein Declines but Its mRNA Increases
during Senescence in Arabidopsis1
L. Michael Weaver,
John E. Froehlich, and
Richard M. Amasino*
Department of Biochemistry, University of Wisconsin, 433 Babcock
Drive, Madison, Wisconsin 53706 (L.M.W., R.M.A.); and Michigan State
University-Department of Energy Plant Research Laboratory, Michigan
State University, East Lansing, Michigan 48823-1312 (J.E.F.)
Arabidopsis ERD1 is a
ClpC-like protein that sequence analysis suggests may interact with the
chloroplast-localized ClpP protease to facilitate proteolysis. The mRNA
encoded by the ERD1 gene has previously been shown to
accumulate in response to senescence and to a variety of stresses and
hormones. Here we show that the ERD1 protein, in contrast to the
ERD1 mRNA, strongly declines in abundance with age,
becoming undetectable in fully expanded leaves. Sequence analysis also
suggests that ERD1 is chloroplast targeted, and we show in an in vitro
system that the native protein is properly imported, processed, and
present within the soluble fraction of the chloroplast, presumably the
stroma. We show that ClpP protein, which is also present in the stroma,
declines with age in parallel with ERD1. These results are consistent
with the interaction of ERD1 and ClpP, but they suggest that it is
unlikely that either plays a major role during senescence. Certain
other chloroplast proteins decline with age coordinately with ERD1 and ClpP, suggesting that these declines are markers of an early
age-mediated change that occurs within the chloroplast.
1
This work was supported through the Consortium
for Plant Biotechnology Research (grant no. DE-FG02-97ER20280) and the
Graduate School of the University of Wisconsin. L.M.W. was partially
supported by National Institutes of Health training grant no. T32
GM07215.
*
Corresponding author; e-mail amasino{at}biochem.wisc.edu; fax
1-608-262-3453.
Plant Physiol. (1999) 119: 1209-1216
Copyright Clearance Center: 0032-0889/99/119//08
© 1999 American Society of Plant Physiologists
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