Plant Physiol. Illumina
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via CrossRef
Right arrow Citing Articles via Web of Science (29)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Chevalier, T.
Right arrow Articles by Fils-Lycaon, B. R.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Chevalier, T.
Right arrow Articles by Fils-Lycaon, B. R.
Agricola
Right arrow Articles by Chevalier, T.
Right arrow Articles by Fils-Lycaon, B. R.

Molecular Cloning and Characterization of Apricot Fruit Polyphenol Oxidase

Tony Chevalier1, David de Rigal1, Didier Mbéguié-A-Mbéguié1, Frédéric Gauillard, Florence Richard-Forget*, and Bernard R. Fils-Lycaon

Institut National de la Recherche Agronomique, Site Agroparc, Domaine Saint Paul, Station de Technologie des Produits Végétaux, 84914 Avignon cedex 9, France (T.C., D.d.R., D.M.-A.-M., F.G., F.R.-F.); and Institut National de la Recherche Agronomique, Domaine Duclos, Station de Technologie des Produits Végétaux, B.P. 515, 97165 Pointe-à-Pitre cedex, French West Indies (B.R.F.-L.)

A reverse transcriptase-polymerase chain reaction experiment was done to synthesize a homologous polyphenol oxidase (PPO) probe from apricot (Prunus armeniaca var Bergeron) fruit. This probe was further used to isolate a full-length PPO cDNA, PA-PPO (accession no. AF020786), from an immature-green fruit cDNA library. PA-PPO is 2070 bp long and contains a single open reading frame encoding a PPO precursor peptide of 597 amino acids with a calculated molecular mass of 67.1 kD and an isoelectric point of 6.84. The mature protein has a predicted molecular mass of 56.2 kD and an isoelectric point of 5.84. PA-PPO belongs to a multigene family. The gene is highly expressed in young, immature-green fruit and is turned off early in the ripening process. The ratio of PPO protein to total proteins per fruit apparently remains stable regardless of the stage of development, whereas PPO specific activity peaks at the breaker stage. These results suggest that, in addition to a transcriptional control of PPO expression, other regulation factors such as translational and posttranslational controls also occur.

1   These authors contributed equally to this work.
*   Corresponding author; e-mail forget{at}avignon.inra.fr; fax 33-04-90-31-62-58.

Plant Physiol. (1999) 119: 1261-1270
Copyright Clearance Center:   0032-0889/99/119//10
© 1999 American Society of Plant Physiologists




This article has been cited by other articles:


Home page
 Plant CellHome page
Y. Yu, T. Tang, Q. Qian, Y. Wang, M. Yan, D. Zeng, B. Han, C.-I Wu, S. Shi, and J. Li
Independent Losses of Function in a Polyphenol Oxidase in Rice: Differentiation in Grain Discoloration between Subspecies and the Role of Positive Selection under Domestication
PLANT CELL, November 1, 2008; 20(11): 2946 - 2959.
[Abstract] [Full Text] [PDF]

Home page
 Food Science and Technology InternationalHome page
E. Chiacchierini, D. Restuccia, and G. Vinci
Bioremediation of Food Industry Effluents: Recent Applications of Free and Immobilised Polyphenoloxidases
Food Science and Technology International, December 1, 2004; 10(6): 373 - 382.
[Abstract] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
M.-H. Cho, S. G. A. Moinuddin, G. L. Helms, S. Hishiyama, D. Eichinger, L. B. Davin, and N. G. Lewis
(+)-Larreatricin hydroxylase, an enantio-specific polyphenol oxidase from the creosote bush (Larrea tridentata)
PNAS, September 16, 2003; 100(19): 10641 - 10646.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
C. P. Constabel, L. Yip, J. J. Patton, and M. E. Christopher
Polyphenol Oxidase from Hybrid Poplar. Cloning and Expression in Response to Wounding and Herbivory
Plant Physiology, September 1, 2000; 124(1): 285 - 296.
[Abstract] [Full Text]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
ASPB Publications PLANT PHYSIOLOGY® THE PLANT CELL
Copyright © 1999 by the American Society of Plant Biologists